Effects of varying fat content of a high tryptophan diet on the induction of gamma-glutamyltranspeptidase positive foci in the livers of rats treated with hepatocarcinogen.

The influence of varying the dietary fat content on the emergence of gamma-glutamyltranspeptidase (GGT)-positive foci in the livers of male rats fed elevated (2%) L-tryptophan (TRP) and exposed to a hepatocarcinogen was investigated. Subtotal hepatectomies were performed, and 18 h later the rats were treated with diethylnitrosamine (DEN) (30 mg/kg). Ten days later 4 dietary groups were followed for 10 weeks: (1) control diet containing 15% fat (C-HF); (2) control diet containing 5% fat (C-LF); (3) C-HF + TRP; (4) C-LF + TRP. Rats fed elevated TRP diets (C-HF + TRP and C-LF + TRP) similarly developed more and larger GGT+ foci than did rats fed the regular TRP diets (C-HF and C-LF), indicating that the promotional effect of elevated dietary TRP was not affected by the fat level (15% vs. 5%).