Liu Xia, Liang Jiu-Ping, Sha Ou, Wang Song-Juan, Li Heng-Guo, Cho Eric Y P
Medical Imaging Center, the First Affiliated Clinical Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China.
School of Biomedical Sciences, the Chinese University of Hong Kong, Shatin, Hong Kong 999077, China.
Int J Ophthalmol. 2017 Jun 18;10(6):854-861. doi: 10.18240/ijo.2017.06.05. eCollection 2017.
To explore if ischemic preconditioning (IPC) can enhance the survival of retinal ganglion cells (RGCs) after optic nerve axotomy.
Twenty-four hours prior to retinal ischemia 60min or axotomy, IPC was applied for ten minutes in groups of (=72) animals. The survival of RGCs, the cellular expression of heat shock protein 27 (HSP27) and heat shock protein 70 (HSP70) and the numbers of retinal microglia in the different groups were quantified at 7 and 14d post-injury. The cellular expression of HSP27 and HSP70 and changes in the numbers of retinal microglia were quantified to detect the possible mechanism of the protection of the IPC.
Ten minutes of IPC promoted RGC survival in both the optic nerve injury (IPC-ONT) and the retinal ischemia 60min (IPC-IR60) groups, examined at 7d and 14d post-injury. Microglial proliferation showed little correlation with the extent of benefit effects of IPC on the rescue of RGCs. The number of HSP27-positive RGCs was significantly higher in the IPC-ONT group than in the sham IPC-ONT group, although the percentage of HSP27-positive RGCs did not significantly differ between groups. For the IPC-IR60 group, neither the number nor the percentage of the HSP27-positive RGCs differed significantly between the IPC and the sham-operated groups. The number of HSP70-positive RGCs was significantly higher for both the IPC-ONT and the IPC-IR60 experimental groups, but the percentages did not differ.
The induction of IPC enhances the survival of RGCs against both axotomy and retinal ischemia.
探讨缺血预处理（IPC）能否提高视神经切断术后视网膜神经节细胞（RGCs）的存活率。
在视网膜缺血60分钟或视神经切断术前24小时，对（=72）只动物分组进行10分钟的IPC处理。在损伤后7天和14天，对不同组中RGCs的存活情况、热休克蛋白27（HSP27）和热休克蛋白70（HSP70）的细胞表达以及视网膜小胶质细胞数量进行定量分析。对HSP27和HSP70的细胞表达以及视网膜小胶质细胞数量的变化进行定量分析，以检测IPC保护作用的可能机制。
在损伤后7天和14天检查发现，10分钟的IPC可促进视神经损伤组（IPC-ONT）和视网膜缺血60分钟组（IPC-IR60）中RGCs的存活。小胶质细胞增殖与IPC对RGCs挽救的有益作用程度几乎没有相关性。IPC-ONT组中HSP27阳性RGCs的数量显著高于假手术IPC-ONT组，尽管各组间HSP27阳性RGCs的百分比无显著差异。对于IPC-IR60组，IPC组和假手术组之间HSP27阳性RGCs的数量和百分比均无显著差异。IPC-ONT和IPC-IR60实验组中HSP70阳性RGCs的数量均显著更高，但百分比无差异。
IPC的诱导可提高RGCs抵抗切断术和视网膜缺血的存活率。
