Liu Bei, Gao Jing, Lyu Bo-Chang, Du Shan-Shuang, Pei Cheng, Zhu Zhong-Qiao, Ma Bo
Shaanxi Ophthalmic Medical Center, Xi'an No.4 Hospital, the Affiliated Guangren Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Department of Ophthalmology, the First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
Int J Ophthalmol. 2017 Jul 18;10(7):1034-1039. doi: 10.18240/ijo.2017.07.03. eCollection 2017.
To investigate the expression differences of transforming growth factor-β2 (TGF-β2), basic fibroblast growth factor (bFGF) and intercellular cell-adhesion molecule-1 (ICAM-1) in lens epithelial cells (LECs) of complicated cataract with silicone oil tamponade and age-related cataract.
Totally 150 eyes of 150 patients (aged 35 to 77y) were investigated, including 75 patients with complicated cataract after silicone oil tamponade and 75 patients with age-related cataract. The central piece of anterior capsules was collected during cataract surgery. TGF-β2, bFGF and ICAM-1 were detected in the 60 specimens of the two groups by immunohistochemistry. The expression levels of the three kinds of messenger ribonucleic acid (mRNA) were determined by real-time quantitative reverse transcription-polymerase chain reaction in the 90 specimens of the two groups.
TGF-β2 was detected in the cytomembrane and cytoplasm of the LECs and bFGF was detected in the nucleus. ICAM-1 was positive in the cytomembrane of the LECs and the distribution of positive cells was uneven. The mRNA genes expression of the TGF-β2, bFGF and ICAM-1 was significant differences between the two groups and markedly increased in complicated cataract group (<0.05).
The up-regulated TGF-β2, bFGF and ICAM-1 maybe associate with the occurrence and development of complicated cataract with silicone oil tamponade.
探讨转化生长因子-β2（TGF-β2）、碱性成纤维细胞生长因子（bFGF）和细胞间细胞黏附分子-1（ICAM-1）在硅油填充复杂白内障和年龄相关性白内障晶状体上皮细胞（LECs）中的表达差异。
对150例患者（年龄35至77岁）的150只眼进行研究，其中包括75例硅油填充后复杂白内障患者和75例年龄相关性白内障患者。白内障手术期间收集前囊中央部分。通过免疫组织化学检测两组60份标本中的TGF-β2、bFGF和ICAM-1。通过实时定量逆转录-聚合酶链反应测定两组90份标本中三种信使核糖核酸（mRNA）的表达水平。
TGF-β2在LECs的细胞膜和细胞质中检测到，bFGF在细胞核中检测到。ICAM-1在LECs的细胞膜中呈阳性，阳性细胞分布不均匀。两组间TGF-β2、bFGF和ICAM-1的mRNA基因表达存在显著差异，且在复杂白内障组中明显升高（<0.05）。
TGF-β2、bFGF和ICAM-1的上调可能与硅油填充复杂白内障的发生和发展有关。
