Liu Yu-Fu, Liu Hong-Wei, Zhou Yi
Beijing Ophthalmology & Visual Sciences Key Lab, Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China.
Int J Ophthalmol. 2010;3(1):58-60. doi: 10.3980/j.issn.2222-3959.2010.01.13. Epub 2010 Mar 18.
To study the differences of basic fibroblast growth factor (bFGF) gene expression in lens epithelial cells (LECs) between fetuses and cataract patients.
In situ hybridization was used to detect bFGF mRNA in the LECs that were cultured and in tissue sections from fetuses and in the LECs from the anterior capsule of cataract patients. Image analysis was used for the relative quantitative analysis of bFGF mRNA.
bFGF gene existed in the LECs that were cultured and in tissue sections from fetuses and in the LECs from the anterior capsule of cataract patients. The integral absorbance for the fetal cultured cells, the fetal tissue sections and the capsule membrane cells of cataract patients were 627.1±268.7, 131.5±42.8 and 79.2±26.3 respectively. The integral absorbance of fetal cultured LECs was significantly higher than that of fetal section LECs (P<0.01). The integral absorbance of cataract LECs was significantly lower than that of fetal LECs (P<0.01).
The in vitro culture of LECs can improve bFGF gene expression. The bFGF gene expression in fetal LECs is significantly higher than that in cataract LECs.
研究胎儿与白内障患者晶状体上皮细胞（LECs）中碱性成纤维细胞生长因子（bFGF）基因表达的差异。
采用原位杂交技术检测培养的LECs、胎儿组织切片以及白内障患者前囊膜LECs中的bFGF mRNA。采用图像分析技术对bFGF mRNA进行相对定量分析。
bFGF基因存在于培养的LECs、胎儿组织切片以及白内障患者前囊膜LECs中。胎儿培养细胞、胎儿组织切片以及白内障患者囊膜细胞的积分吸光度分别为627.1±268.7、131.5±42.8和79.2±26.3。胎儿培养的LECs的积分吸光度显著高于胎儿切片LECs（P<0.01）。白内障LECs的积分吸光度显著低于胎儿LECs（P<0.01）。
LECs的体外培养可提高bFGF基因表达。胎儿LECs中的bFGF基因表达显著高于白内障LECs。
