Emanuelli G, Anfossi G, Calcamuggi G, Marcarino C, Ottone G, Dughera L
Enzyme. 1985;34(4):177-85. doi: 10.1159/000469383.
Blood lithium (Li) levels, renal functional parameters and urine excretion of enzymatic activities having different intracellular sites were investigated on rats submitted to acute and subacute Li chloride administration. In acute experiments increased levels of all detected enzymes were assayed following Li single doses of 5 and 10 mEq/kg b.w. In subacute poisoning, urine output of lactate dehydrogenase, aspartate transaminase and alanine transaminase was significantly over the basal ranges following 15 days in concomitance with marked elevation of plasma Li levels and exhibited progressive increase until 30 days; on the 10th day following Li withdrawal, elevated excretion of enzymatic activities was still assayed. The results are in agreement with data about the localization of the histologic lesions involving different sites of the nephron in acute Li poisoning and the distal tubular tract in subacute toxicity. In subacute administration the output of cytoplasmic and mitochondrial activities can be assumed as an index of damage of the nephron cells which can persist following Li withdrawal. Our findings indicate that the urine enzyme assay is a valuable tool to detect renal damage in experimental Li nephropathy.
对接受急性和亚急性氯化锂给药的大鼠，研究了血锂（Li）水平、肾功能参数以及具有不同细胞内定位的酶活性的尿排泄情况。在急性实验中，单次给予5和10 mEq/kg体重的氯化锂后，检测到所有酶的水平均升高。在亚急性中毒时，伴随血浆锂水平显著升高，乳酸脱氢酶、天冬氨酸转氨酶和丙氨酸转氨酶的尿量在15天后明显高于基础范围，并持续增加直至30天；在停锂后的第10天，仍检测到酶活性排泄增加。这些结果与急性锂中毒时涉及肾单位不同部位以及亚急性毒性时远端肾小管的组织学损伤定位数据一致。在亚急性给药时，细胞质和线粒体活性的输出可被视为肾单位细胞损伤的指标，这种损伤在停锂后仍会持续。我们的研究结果表明，尿酶测定是检测实验性锂肾病肾损伤的有价值工具。
