Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
Future Industries Institute, University of South Australia, Mawson Lakes, Australia.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2018 May;10(3):e1486. doi: 10.1002/wnan.1486. Epub 2017 Jul 21.
How to study nanoparticle-cell interactions is the key question that puzzles researchers in the fields of nanomedicine as well as in nanotoxicology. In nanotoxicology, the amount of nanoparticles internalized by the cells or bound to the external surfaces of cells determines the toxic profile of those particles. In medical applications, cellular uptake and binding of medically effective nanoparticles decides their efficacy. Despite the importance of understanding the extent and mode of nanoparticle-cell interactions, these processes are underinvestigated, mainly due to the lack of suitable user-friendly methodologies. Here we discuss the advantages and limitations of currently available (and most advanced) microscopic, spectroscopic, and other bioanalytical methods that could be used to assess cell-nanoparticle interactions either qualitatively or quantitatively. Special emphasis is given to the methods that enable analysis and identification of nanoparticles at single-cell level, and allow intracellular localization and speciation analysis of nanoparticles. This article is categorized under: Nanotechnology Approaches to Biology > Cells at the Nanoscale Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials.
如何研究纳米颗粒与细胞的相互作用是纳米医学和纳米毒理学领域研究人员感到困惑的关键问题。在纳米毒理学中，细胞内化或结合到细胞外部表面的纳米颗粒的数量决定了这些颗粒的毒性特征。在医学应用中，医学有效纳米颗粒的细胞摄取和结合决定了它们的疗效。尽管了解纳米颗粒与细胞相互作用的程度和模式非常重要，但这些过程仍未得到充分研究，主要是由于缺乏合适的用户友好型方法。在这里，我们讨论了目前可用的（最先进的）微观、光谱和其他生物分析方法的优缺点，这些方法可用于定性或定量地评估细胞-纳米颗粒相互作用。特别强调了能够在单细胞水平上分析和识别纳米颗粒的方法，以及允许对纳米颗粒进行细胞内定位和形态分析的方法。本文属于以下类别： 生物学中的纳米技术方法 > 纳米尺度下的细胞 纳米医学中的毒理学和监管问题 > 纳米材料的毒理学
