Kosik K S, Bakalis S F, Selkoe D J, Pierce M W, Duffy L K
J Neurosci Res. 1986;15(4):543-51. doi: 10.1002/jnr.490150411.
The high molecular weight microtubule-associated proteins (MAPs) consist of MAPs 1A, 1B, 1C, 2A, and 2B. The proteins have been prepared from rat brain by using taxol and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The individual proteins were purified by electro-elution from stained fixed gels and shown to migrate at their original mobility without proteolysis. Amino acid compositions of purified MAP 1 and 2 component proteins are reported and compared.
高分子量微管相关蛋白（MAPs）由MAPs 1A、1B、1C、2A和2B组成。这些蛋白是通过使用紫杉醇从大鼠脑中制备出来的，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离。各个蛋白通过从染色的固定凝胶中电洗脱进行纯化，并显示在没有蛋白水解的情况下以其原始迁移率迁移。报告并比较了纯化的MAP 1和2组分蛋白的氨基酸组成。
