Peng I, Binder L I, Black M M
Brain Res. 1985 Dec 30;361(1-2):200-11. doi: 10.1016/0006-8993(85)91290-9.
The non-tubulin proteins associated with microtubules (MAPs) in cultures of pure sympathetic neurons have been identified using a variety of biochemical and immunochemical methods. MAPs of cultured sympathetic neurons include proteins corresponding to brain MAP-1 (consisting of MAP-1a and MAP-1b species), MAP-2, MAP-3, tau, 4 proteins that range in molecular weight from 60,000 to 76,000, and proteins with molecular weights of 210,000, 130,000 and 32,000. Many of the MAPs are phosphorylated in situ. MAP-2 and tau of cultured sympathetic neurons differ from their counterparts of brain in electrophoretic mobility. The observed variety of MAPs in sympathetic neurons together with the differences in MAPs of brain and sympathetic neurons are discussed in terms of microtubule heterogeneity in the nervous system.
利用多种生化和免疫化学方法，已在纯交感神经元培养物中鉴定出与微管相关的非微管蛋白（MAPs）。培养的交感神经元的MAPs包括与脑MAP-1（由MAP-1a和MAP-1b种类组成）、MAP-2、MAP-3、tau相对应的蛋白质，4种分子量在60,000至76,000之间的蛋白质，以及分子量为210,000、130,000和32,000的蛋白质。许多MAPs在原位被磷酸化。培养的交感神经元的MAP-2和tau在电泳迁移率方面与其脑内对应物不同。从神经系统中微管的异质性角度讨论了在交感神经元中观察到的MAPs多样性以及脑和交感神经元MAPs的差异。
