Department of Internal Medicine, Chi Mei Medical Center, Tainan City, Taiwan; Chia Nan University of Pharmacy & Science, Tainan City, Taiwan.
Department of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
J Microbiol Immunol Infect. 2018 Dec;51(6):770-777. doi: 10.1016/j.jmii.2017.02.003. Epub 2017 Jun 28.
BACKGROUND/PURPOSE: Globally, multidrug-resistant Acinetobacter baumannii (MDRAB) has emerged as an important pathogen in nosocomial outbreaks. This study aimed to investigate the correlation between the biofilm formation and survival of MDRABs, and to investigate the antiseptic efficacy of hand sanitizers for the MDRABs, embedded with biofilm (MDRAB-Bs).
The MDRABs were selected randomly after pulsed-field gel electrophoresis (PFGE), and their biofilm formation was analyzed. Desiccation and ethanol tolerances were assayed to test the bacterial survival. The antiseptic efficacy of combined chlorhexidine gluconate (CHG) and 70% ethanol agents against MDRAB-Bs were compared with the 70% ethanol cleanser.
Eleven MDRABs, which varied in biofilm formation (MRDAB-B) and planktonic type (MDRAB-P), were tested. In desiccation survival, the mean survival time for the MDRAB-Bs was 49.0 days which was significantly higher than that of their planktonic type (17.3 days) (P < 0.005). The MDRAB-Ps could be eliminated after a 10 min contact with a 30% ethanol agent, however, it took 10 min of 70% ethanol to eliminate the MDRAB-Bs. On the other hand, a 2% CHG in 70% ethanol solution completely eliminated all MDRAB-Bs after 1 min contacted time. The 2% CHG in 70% ethanol agent provided a significantly superior efficacy than the 70% ethanol solution at eliminating the MDRAB-Bs (P < 0.005).
MDRAB with biofilm-formation presented significantly higher desiccation and ethanol resistances than their planktonic type. Moreover, the 2% CHG in 70% ethanol agent provided a superior antiseptic efficacy for MDRAB-Bs than that of the 70% ethanol agent.
背景/目的：在全球范围内，耐多药鲍曼不动杆菌（MDRAB）已成为医院感染爆发的重要病原体。本研究旨在探讨 MDRAB 生物膜形成与存活之间的相关性，并研究含生物膜（MDRAB-Bs）的 MDRAB 对手卫生消毒剂的消毒效果。
通过脉冲场凝胶电泳（PFGE）随机选择 MDRAB，并分析其生物膜形成情况。通过干燥和乙醇耐受试验来检测细菌的存活情况。比较了含葡萄糖酸氯己定（CHG）和 70%乙醇的联合制剂与 70%乙醇清洁剂对 MDRAB-Bs 的消毒效果。
共检测了 11 株 MDRAB，它们在生物膜形成（MRDAB-B）和浮游型（MDRAB-P）方面存在差异。在干燥存活方面，MDRAB-Bs 的平均存活时间为 49.0 天，明显高于其浮游型（17.3 天）（P<0.005）。MDRAB-Ps 在 10 分钟接触 30%乙醇后即可被消除，但消除 MDRAB-Bs 需要 10 分钟接触 70%乙醇。另一方面，2% CHG 在 70%乙醇溶液中，接触 1 分钟即可完全消除所有 MDRAB-Bs。2% CHG 在 70%乙醇溶液中的消毒效果明显优于 70%乙醇溶液（P<0.005）。
具有生物膜形成的 MDRAB 比其浮游型具有更高的干燥和乙醇抗性。此外，2% CHG 在 70%乙醇溶液中的消毒效果优于 70%乙醇溶液。
