Betchen Melanie, Giovinco Holly M, Curry Michael, Luu Jackson, Fraimow Henry, Carabetta Valerie J, Nahra Raquel
Department of Internal Medicine, Cooper University Hospital, Camden, NJ 08103, USA.
Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ 08103, USA.
Antibiotics (Basel). 2022 May 3;11(5):614. doi: 10.3390/antibiotics11050614.
hospital infections are difficult to treat due to the rapid emergence of multidrug-resistant (MDR) strains. In addition, can survive in numerous adverse environments, including in the presence of common hospital antiseptics. We hypothesized that in addition to accumulating drug resistance determinants, MDR strains also accumulate mutations that allow for greater microbicide tolerance when compared to pan-susceptible (PS) strains. To test this hypothesis, we compared the survival of five MDR and five PS patient isolates when exposed to bleach, ethanol, quaternary ammonium compounds, chlorhexidine gluconate, and povidone. We evaluated bacteria in a free-living planktonic state and under biofilm conditions. Each disinfectant eliminated 99.9% of planktonic bacteria, but this was not the case for bacterial biofilms. Next, we characterized strains for the presence of the known microbicide-resistance genes , , , and . MDR strains did not survive more than PS strains in the presence of microbicides, but microbicide-resistant strains had higher survival rates under some conditions. Interestingly, the PS strains were more likely to possess microbicide-resistance genes. Microbicide resistance remains an important topic in healthcare and may be independent of antimicrobial resistance. Hospitals should consider stricter isolation precautions that take pan-susceptible strains into account.
由于多重耐药（MDR）菌株的迅速出现，医院感染难以治疗。此外，（此处原文有缺失信息）能够在多种不利环境中存活，包括在常见医院消毒剂存在的情况下。我们推测，与泛敏感（PS）菌株相比，MDR菌株除了积累耐药决定因素外，还积累了能使其对杀菌剂具有更高耐受性的突变。为了验证这一假设，我们比较了5株MDR患者分离株和5株PS患者分离株在接触漂白剂、乙醇、季铵化合物、葡萄糖酸洗必泰及聚维酮碘时的存活率。我们评估了处于自由生活浮游状态和生物膜条件下的细菌。每种消毒剂都能消除99.9%的浮游细菌，但对细菌生物膜则不然。接下来，我们对菌株进行了已知抗微生物剂抗性基因（此处原文有缺失信息）的鉴定。在存在杀菌剂的情况下，MDR菌株的存活情况并不比PS菌株好，但在某些条件下，抗杀菌剂菌株的存活率更高。有趣的是，PS菌株更有可能拥有抗杀菌剂基因。抗杀菌剂仍然是医疗保健中的一个重要课题，并且可能与抗菌耐药性无关。医院应考虑采取更严格的隔离预防措施，将泛敏感菌株考虑在内。
