Department of Pharmacy, Clinical Medical College and The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China.
Department of Pharmacy, Mianyang Central Hospital, Mianyang, Sichuan, China.
BMC Infect Dis. 2021 Mar 17;21(1):266. doi: 10.1186/s12879-021-05963-6.
Chlorhexidine is a widely used disinfectant in clinical settings and a broad-spectrum antimicrobial agent effective against aerobic and anaerobic bacteria. However, disinfectant resistant or non-susceptible bacteria, including antibiotic-resistant Acinetobacter baumannii, have been found. This study aimed to develop a new technique to prevent and control A. baumannii infection in the hospital setting.
Chlorhexidine combined with minocycline, doxycycline, meropenem, imipenem, levofloxacin and ciprofloxacin were tested against the 30 multidrug-resistant and extremely drug-resistant A. baumannii clinical isolates. The checkerboard test was used to calculate the fractional inhibitory concentration index according to the minimum inhibitory concentration value for chlorhexidine combined with antibiotics.
The combination of chlorhexidine with minocycline, doxycycline, meropenem, or ciprofloxacin showed synergistic responses in all clinical isolates, and more than 50% of isolates showed FICI ≤0.5. However, chlorhexidine together with imipenem or levofloxacin showed indifferent responses in 10% and 3.33% clinical isolates, respectively. In all tests, combinations of chlorhexidine with each of the above six antibiotics showed synergistic and additive effects, and inhibited the clinical isolates.
We concluded that, chlorhexidine combined with antibiotics could be used to control the risk of infection with A. baumannii.
洗必泰是一种在临床环境中广泛使用的消毒剂，也是一种广谱抗菌剂，对需氧菌和厌氧菌均有作用。然而，已经发现了具有抗消毒剂性或非敏感性的细菌，包括抗生素耐药的鲍曼不动杆菌。本研究旨在开发一种新的技术，以预防和控制医院环境中的鲍曼不动杆菌感染。
用洗必泰联合米诺环素、多西环素、美罗培南、亚胺培南、左氧氟沙星和环丙沙星对 30 株多药耐药和极耐药鲍曼不动杆菌临床分离株进行测试。根据洗必泰联合抗生素的最低抑菌浓度值，用棋盘试验计算部分抑菌浓度指数。
在所有临床分离株中，洗必泰与米诺环素、多西环素、美罗培南或环丙沙星联合均表现出协同作用，超过 50%的分离株的 FICI≤0.5。然而，洗必泰与亚胺培南或左氧氟沙星联合在 10%和 3.33%的临床分离株中分别表现出无关反应。在所有试验中，洗必泰与上述六种抗生素中的每一种联合均表现出协同和相加作用，并抑制了临床分离株。
我们得出结论，洗必泰联合抗生素可用于控制鲍曼不动杆菌感染的风险。
