Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
RESCOLL Société de Recherche, Allée Geoffroy Saint Hilaire 8, F-33615 Pessac, France.
Carbohydr Polym. 2017 Oct 1;173:482-488. doi: 10.1016/j.carbpol.2017.06.030. Epub 2017 Jun 9.
Sterilization methods such as ɣ-irradiation, steam sterilization and ethylene oxide gas treatment can have negative effects on molecular structure and properties of polysaccharide-based biomaterials. In this perspective, the use of supercritical carbon dioxide (scCO) has been proposed as an alternative method for biomaterial sterilization. In this work, chemical, mechanical and biological properties of polysaccharidic membranes for surgical applications were investigated after sterilization by scCO. Four sets of sterilizing conditions were considered and SEC analyses were performed in order to identify the one with lower impact on the polysaccharidic matrix of membranes (alginate). Mechanical tests showed that the resistance of membranes was slightly affected after sterilization. Biological analyses proved the biocompatibility of the sterilized membranes both in vitro and in a preliminary in vivo test. Overall, this study points out that this sterilization technique can be successfully employed to achieve an effective and safe sterilization of polysaccharidic membranes for surgical use.
辐照、蒸汽灭菌和环氧乙烷气体处理等灭菌方法可能对基于多糖的生物材料的分子结构和性能产生负面影响。在这种情况下，已经提出使用超临界二氧化碳（scCO）作为生物材料灭菌的替代方法。在这项工作中，研究了用于外科应用的多糖膜的化学、机械和生物学性质，这些膜经过 scCO 灭菌。考虑了四组灭菌条件，并进行了 SEC 分析，以确定对膜（海藻酸盐）的多糖基质影响较小的条件。机械测试表明，灭菌后膜的阻力略有受影响。生物学分析证明了灭菌膜在体外和初步体内试验中的生物相容性。总的来说，这项研究指出，这种灭菌技术可成功用于实现外科用多糖膜的有效和安全灭菌。
