Zhang Xinrui, Le Linh Thi Thuy, Jin Yongxun, Jin Caijun, Giang Nguyen Ngan, Trinh Thuy-Tien Thi, Lee Yong Hyun, Shin Yong Woo, Bae Jin Woo, Chien Pham Ngoc, Heo Chan Yeong
Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea.
Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.
Int J Mol Sci. 2025 Jun 14;26(12):5715. doi: 10.3390/ijms26125715.
Wound healing remains a significant clinical challenge worldwide, and effective management strategies are essential for improving outcomes. This study evaluated SCderm Matrix, a novel acellular dermal matrix (ADM) patch developed using supercritical carbon dioxide (sCO) processing of human skin tissue. This innovative processing method preserves structural integrity while enhancing biocompatibility, resulting in a patch characterized by porous architecture, uniform thickness, excellent tensile strength, and optical transparency. In vivo wound healing experiments using full-thickness skin wounds in Sprague-Dawley rats demonstrated the patch's superior performance. Treatment with the sCO ADM patch accelerated wound closure, reduced inflammation, and enhanced granulation tissue formation compared to both untreated controls and two commercially available ADM products. Histological analysis revealed improved re-epithelialization and collagen deposition, while molecular and immunohistochemical assessments showed decreased reactive oxygen species (ROS) and pro-inflammatory cytokines. Simultaneously, the treatment upregulated key proliferation and remodeling markers including alpha smooth muscle actin (α-SMA), vimentin, and transforming growth factor beta 1 (TGF-β1). These findings demonstrate that the SCderm Matrix promotes wound healing through multiple mechanisms: modulating inflammatory responses, enhancing antioxidant defenses, and supporting tissue regeneration. The results suggest this biomaterial has significant potential as an effective and versatile solution for clinical wound care applications.
伤口愈合在全球范围内仍然是一项重大的临床挑战，有效的管理策略对于改善治疗效果至关重要。本研究评估了SCderm Matrix，这是一种新型的无细胞真皮基质（ADM）贴片，它是通过对人类皮肤组织进行超临界二氧化碳（sCO）处理而开发的。这种创新的处理方法在增强生物相容性的同时保持了结构完整性，从而形成了一种具有多孔结构、厚度均匀、拉伸强度优异和光学透明性的贴片。在Sprague-Dawley大鼠身上使用全层皮肤伤口进行的体内伤口愈合实验证明了该贴片的卓越性能。与未处理的对照组和两种市售ADM产品相比，使用sCO ADM贴片治疗加速了伤口闭合，减轻了炎症，并增强了肉芽组织形成。组织学分析显示上皮再形成和胶原蛋白沉积得到改善，而分子和免疫组织化学评估显示活性氧（ROS）和促炎细胞因子减少。同时，该治疗上调了关键的增殖和重塑标志物，包括α平滑肌肌动蛋白（α-SMA）、波形蛋白和转化生长因子β1（TGF-β1）。这些发现表明，SCderm Matrix通过多种机制促进伤口愈合：调节炎症反应、增强抗氧化防御和支持组织再生。结果表明，这种生物材料作为临床伤口护理应用的一种有效且通用的解决方案具有巨大潜力。
