College of Marine Life Science, Ocean University of China, Yushan Road, No. 5, Qingdao, Shandong 266003, China.
College of Marine Life Science, Ocean University of China, Yushan Road, No. 5, Qingdao, Shandong 266003, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Carbohydr Polym. 2017 Oct 1;173:592-599. doi: 10.1016/j.carbpol.2017.06.043. Epub 2017 Jun 12.
A convenient and efficient two-stage bioprocess was established for fructo-oligosaccharides (FOSs) production, during which the endo-inulinase was first produced and subsequently the inulin supplemented was directly hydrolyzed by the produced endo-inulinase, in the meantime the generated non-prebiotic saccharides was assimilated by the yeast cells. This process was implemented by an engineered Yarrowia lipolytica strain Enop56, in which an optimized endo-inulinase gene from Aspergillus niger was overexpressed. When the strain Enop56 was fermented with an inulin concentration of 600g/L in a 10-L bioreactor, the inulinase activity, FOSs titer, yield and productivity reached to 551.6U/mL, 546.6g/L, 0.91g/g, and 15.18g/L/h, respectively. Besides, the hydrolysis products were mainly FOSs with polymerization degrees of 3-5 and the total amount of non-prebiotic mono- and disaccharides was only 4.97% in the final fermentation broth. This study demonstrated that the two-stage bioprocess using the strain Enop56 was a promising strategy to produce FOSs on an industrial scale.
建立了一种方便、高效的两步法生物工艺来生产果寡糖（FOSs），在此过程中，首先生产内切菊粉酶，然后用所产生的内切菊粉酶直接水解菊粉，同时酵母细胞将产生的非益生元糖吸收。该过程由工程化的解脂耶氏酵母菌株 Enop56 实现，其中过表达了来自黑曲霉的优化内切菊粉酶基因。当菌株 Enop56 在 10 升生物反应器中发酵 600g/L 的菊粉时，菊粉酶活性、FOSs 浓度、产率和生产效率分别达到 551.6U/mL、546.6g/L、0.91g/g 和 15.18g/L/h。此外，水解产物主要是聚合度为 3-5 的 FOSs，最终发酵液中非益生元单糖和二糖的总量仅为 4.97%。本研究表明，使用 Enop56 菌株的两步法生物工艺是一种在工业规模上生产 FOSs 的有前途的策略。
