精胺改性淀粉基载体用于基因传递：结构-转染活性关系。
Spermine modified starch-based carrier for gene delivery: Structure-transfection activity relationships.
机构信息
Ministry of Education Engineering Research Center of Starch & Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China; Department of Chemistry, Materials, and Chemical Engineering "G.Natta", Politecnico di Milano, Milan 20131, Italy.
Ministry of Education Engineering Research Center of Starch & Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.
出版信息
Carbohydr Polym. 2017 Oct 1;173:690-700. doi: 10.1016/j.carbpol.2017.05.099. Epub 2017 Jun 4.
This study aims at developing starch based gene carriers with low cytotoxicity and high transfection efficiency. Starch molecules with molecular weight of about 50kDa were cationically modified by spermine to obtain spermine modified starch (SMS) based gene carriers. Plasmid pAcGFP1-C1 (pDNA) was chosen as the model gene material and formed self-assembly nanocomplexes with SMS. The cytotoxicity and transfection efficiency of SMS/pDNA complexes were tested in HepG2 cell lines. Results showed that SMS/pDNA complexes formed by SMS-DS3 with the highest primary amine content (1.17μmolmg) and pDNA at the weight ratio of 25 displayed the highest transfection efficiency (∼40%) with low cytotoxicity. Dynamic Light Scattering (DLS) and Small Angel X-ray Scattering (SAXS) investigation under simulated acidified endosomal environments (pH 5.0-7.4) revealed that SMS-DS3/pDNA complexes (∼180nm) with moderately compact structures within acidic environments (i.e. no significant particle size changes, slightly shrunken shapes) showed superior transfection efficiency.
本研究旨在开发低细胞毒性和高转染效率的基于淀粉的基因载体。采用 spermine 对分子量约为 50kDa 的淀粉分子进行阳离子改性，得到基于 spermine 改性淀粉（SMS）的基因载体。选择质粒 pAcGFP1-C1（pDNA）作为模型基因材料，并与 SMS 形成自组装纳米复合物。在 HepG2 细胞系中测试了 SMS/pDNA 复合物的细胞毒性和转染效率。结果表明，具有最高伯胺含量（1.17μmolmg）的 SMS-DS3 与 pDNA 的重量比为 25 时，SMS/pDNA 复合物的转染效率最高（约 40%），细胞毒性较低。在模拟酸化内涵体环境（pH 5.0-7.4）下进行动态光散射（DLS）和小角 X 射线散射（SAXS）研究表明，具有适度紧凑结构的 SMS-DS3/pDNA 复合物（约 180nm）在酸性环境下（即无明显粒径变化，形状略有收缩）表现出优异的转染效率。
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