School of Chemical Sciences, Central University of Gujarat, Gandhinagar 382030, India.
School of Nano Scences, Central University of Gujarat, Gandhinagar 382030, India.
Ultrason Sonochem. 2017 Nov;39:208-217. doi: 10.1016/j.ultsonch.2017.04.006. Epub 2017 Apr 7.
The non-hazardous sonochemical approach has been developed for the functionalization of graphene oxide (GrO) with 5-Aminoindazole (5-AIND). The formation of f-(5-AIND) GrO is confirmed with C solid state NMR, HRXPS, XRD, Raman, TGA, DSC, FTIR etc. The >80% cell viabilities on MCF-7 and Vero cell lines have confirmed the high cytocompatibility of f-(5-AIND) GrO. Additionally, the morphological impact on Vero cell line has evidently confirmed the biocompatibility of f-(5-AIND) GrO. As compared to GrO, the f-(5-AIND) GrO has demonstrated an enhanced antioxidant efficacy of 69.4-72%, evaluated with 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay. With a similar objective (0.01-0.05)mL peanut oil based curcumin micro and nanoemulsions have been prepared using ethanol and glycerol as co-solvent and co-surfactant respectively. The prepared emulsions are subsequently characterised with respect to morphological and physicochemical parameters via density, surface tension, viscosity, friccohesity measurement and DLS analysis. Henceforth, with an expectation to achieve higher dispersion, an ethanolic dispersion of f-(5-AIND) GrO has been mixed with curcumin carrying emulsions in 1:1. Notably, the radical scavenging activities (RSA) of the combined formulations are significantly enhanced to an extent of 26.6%.
采用无危险的声化学方法将 5-氨基吲唑（5-AIND）功能化氧化石墨烯（GrO）。C 固体核磁共振、高分辨率 X 射线光电子能谱、X 射线衍射、拉曼、热重分析、差示扫描量热法、傅里叶变换红外光谱等证实了 f-(5-AIND)GrO 的形成。MCF-7 和 Vero 细胞系的细胞存活率>80%，证实了 f-(5-AIND)GrO 的高细胞相容性。此外，对 Vero 细胞系的形态影响明显证实了 f-(5-AIND)GrO 的生物相容性。与 GrO 相比，f-(5-AIND)GrO 在 2,2-二苯基-1-苦基肼（DPPH）自由基测定中表现出增强的抗氧化功效为 69.4-72%。为了达到相同的目的（0.01-0.05）mL 基于花生油的姜黄素微乳液和纳米乳液分别使用乙醇和甘油作为共溶剂和共表面活性剂制备。随后通过密度、表面张力、粘度、 friccohesity 测量和 DLS 分析对制备的乳液进行形态和物理化学参数的表征。此后，为了实现更高的分散性，将 f-(5-AIND)GrO 的乙醇分散体与载有姜黄素的乳液以 1:1 的比例混合。值得注意的是，组合制剂的自由基清除活性（RSA）显著增强，达到 26.6%。
