Li Pengliang, Dai Weidong, Lu Meiling, Xie Dongchao, Tan Junfeng, Yang Chen, Zhu Yin, Lv Haipeng, Peng Qunhua, Zhang Yue, Guo Li, Ni Dejiang, Lin Zhi
Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, Zhejiang, People's Republic of China.
College of Horticulture and Forestry Science, Huazhong Agricultural University, Hongshan District, Wuhan, Hubei Province, People's Republic of China.
J Sci Food Agric. 2018 Feb;98(3):1153-1161. doi: 10.1002/jsfa.8566. Epub 2017 Aug 30.
Green tea and black tea are manufactured using appropriate tea cultivars in China. However, the metabolite differences relating to the manufacturing suitability of tea cultivars are unclear. In the present study, we performed a non-targeted metabolomic analysis on 13 Chinese tea cultivars using ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry to investigate comprehensively the metabolite differences between cultivars suitable for manufacturing green tea (GT cultivars) and cultivars suitable for manufacturing both green tea and black tea (G&BT cultivars).
Multivariate statistical analysis and cluster analysis divided the 13 cultivars into two groups, namely GT cultivars and G&BT cultivars, which correlated with their manufacturing suitability. The GT cultivars contained higher levels of flavonoid glycosides, whereas the G&BT cultivars contained higher levels of catechins, dimeric catechins, phenolic acids and alkaloids.
Metabolic pathway analysis revealed that the flavonoid pathway inclined toward the synthesis of flavonoid glycosides in GT cultivars, whereas it inclined toward the synthesis of catechins and phenolic acids in G&BT cultivars. The results of the present study will be helpful for discriminating the manufacturing suitability of tea cultivars and investigating their breeding. © 2017 Society of Chemical Industry.
在中国，绿茶和红茶是使用适宜的茶树品种加工而成的。然而，与茶树品种加工适宜性相关的代谢物差异尚不清楚。在本研究中，我们使用超高效液相色谱联用四极杆-飞行时间质谱，对13个中国茶树品种进行了非靶向代谢组学分析，以全面研究适合加工绿茶的品种（绿茶品种）和适合加工绿茶及红茶的品种（绿&红茶品种）之间的代谢物差异。
多变量统计分析和聚类分析将这13个品种分为两组，即绿茶品种和绿&红茶品种，这与它们的加工适宜性相关。绿茶品种含有较高水平的黄酮苷，而绿&红茶品种含有较高水平的儿茶素、二聚体儿茶素、酚酸和生物碱。
代谢途径分析表明，黄酮途径在绿茶品种中倾向于黄酮苷的合成，而在绿&红茶品种中倾向于儿茶素和酚酸的合成。本研究结果将有助于鉴别茶树品种的加工适宜性并研究其育种。© 2017化学工业协会。
