Moroni F, Luzzi S, Franchi-Micheli S, Zilletti L
Neurosci Lett. 1986 Jul 11;68(1):57-62. doi: 10.1016/0304-3940(86)90229-6.
The actions of agonists and antagonists of excitatory amino acid receptors were studied in the isolated ileal longitudinal muscle-myenteric plexus preparation of the guinea pig incubated 'in vitro', by recording the contraction of the longitudinal muscle. L-Glutamate, L-aspartate, quinolinate and N-methyl-D-aspartate (NMDA), in concentrations ranging from 10(-6) to 10(-4) M, induced a rapid contraction of this preparation while kainate and quisqualate were not active at a concentration of 10(-4) M. The excitatory amino acid responses were competitively antagonized by 2-amino-5-phosphonovalerate. They were also prevented by Mg2+ ions (0.1-1 mM), by tetrodotoxin 3 X 10(-6) M and by hyoscine 10(-7) M. The last observations suggest that the myenteric cholinergic interneurons are in some way involved in the glutamate-induced ileal contraction. These results demonstrate the existence of receptors for excitatory amino acids (possibly of NMDA type) in the myenteric plexus of the guinea pig.
通过记录豚鼠离体回肠纵行肌-肌间神经丛标本中纵行肌的收缩，对兴奋性氨基酸受体激动剂和拮抗剂的作用进行了体外研究。L-谷氨酸、L-天冬氨酸、喹啉酸和N-甲基-D-天冬氨酸（NMDA），浓度范围为10(-6)至10(-4)M时，可引起该标本快速收缩，而在10(-4)M浓度下，海人藻酸和quisqualate无活性。兴奋性氨基酸反应被2-氨基-5-磷酸戊酸竞争性拮抗。它们也被Mg2+离子（0.1-1 mM）、3×10(-6)M的河豚毒素和10(-7)M的东莨菪碱所阻断。最后的观察结果表明，肌间胆碱能中间神经元在某种程度上参与了谷氨酸诱导的回肠收缩。这些结果证明豚鼠肌间神经丛中存在兴奋性氨基酸受体（可能是NMDA型）。
