Sinský M, Donnerer J
Institute of Experimental and Clinical Pharmacology, University of Graz, Austria.
Neurosci Lett. 1998 Dec 18;258(2):109-12. doi: 10.1016/s0304-3940(98)00866-0.
Longitudinal muscle-myenteric plexus strips of the guinea pig ileum were used to investigate a possible transmitter role of glutamate (Glu) in the enteric nervous system. Glu was released from this nerve muscle preparation by high K(+)-depolarization in a Ca2(+)-dependent manner, by electrical field stimulation and by the ganglionic stimulant dimethylphenylpiperazinium which indicates its neural origin. Contractions of the longitudinal muscle evoked by electrical stimulation of the myenteric nerves or by Glu, were significantly reduced by the N-methyl-D-aspartate (NMDA)-receptor antagonist FR115427 (9 and 18 microM), whereas contractions induced by histamine were unaffected. The results show that the amino acid Glu is likely to play an excitatory neurotransmitter role via NMDA receptors in the myenteric plexus of the guinea pig.
采用豚鼠回肠纵行肌-肌间神经丛条来研究谷氨酸（Glu）在肠神经系统中可能作为递质的作用。在该神经肌肉标本中，高钾（K⁺）去极化以钙（Ca²⁺）依赖的方式、通过电场刺激以及神经节兴奋剂二甲基苯基哌嗪使Glu释放，这表明其来源于神经。N-甲基-D-天冬氨酸（NMDA）受体拮抗剂FR115427（9和18微摩尔）可显著降低由肌间神经电刺激或Glu诱发的纵行肌收缩，而组胺诱发的收缩则不受影响。结果表明，氨基酸Glu可能通过NMDA受体在豚鼠肌间神经丛中发挥兴奋性神经递质的作用。
