Chen Weina, Luu Hung S
Department of Pathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, USA.
Department of Pathology, Children's Medical Center at Dallas, Univeristy of Texas Southwestern Medical Center, Dallas, TX, USA.
Methods Mol Biol. 2017;1633:51-73. doi: 10.1007/978-1-4939-7142-8_4.
Multiparameter flow cytometry has become an indispensable tool for the diagnosis and classification of acute myeloid leukemia (AML). The basic method relies on the unique ability to detect immunophenotypic abnormalities on discrete subpopulations. The primary roles in the initial assessment of AML are to determine the immaturity of the leukemic population, define the lineage and the immunophenotypic aberrancies in blasts, and identify characteristic immunophenotypic features to predict important recurrent cytogenetic and genetic abnormalities and prognosis. The established immunophenotypic profile, a baseline "fingerprint," is used for follow-up assessment of residual disease. This chapter provides an overview of procedures for specimen processing, staining, and immunophenotyping of AML and describes our strategy for data analysis supplemented with illustrative case examples.
多参数流式细胞术已成为急性髓系白血病（AML）诊断和分类中不可或缺的工具。基本方法依赖于检测离散亚群上免疫表型异常的独特能力。AML初始评估的主要作用是确定白血病细胞群的不成熟程度，定义原始细胞的谱系和免疫表型异常，并识别特征性免疫表型特征以预测重要的复发性细胞遗传学和基因异常及预后。已建立的免疫表型谱，即基线“指纹”，用于残留疾病的随访评估。本章概述了AML标本处理、染色和免疫表型分析的程序，并描述了我们的数据分析策略，并辅以实例说明。
