Galera Pallavi Kanwar, Jiang Chunjie, Braylan Raul
Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health (NIH), Bethesda, MD, USA.
Methods Mol Biol. 2019;2032:281-296. doi: 10.1007/978-1-4939-9650-6_15.
Immunophenotyping by multiparameter flow cytometry is a rapid and efficient technique to simultaneously assess and correlate multiple individual cell properties like size and internal complexity along with antigen expression in a population of cells. This method is utilized for rapid characterization of the blasts and classification of acute myeloid leukemia (AML), in both the peripheral blood (PB) and bone marrow (BM). This technique is not only useful in the initial diagnosis but also in monitoring and determining prognosis of the disease through minimal residual disease (MRD) testing. This chapter provides an overview of procedures for specimen processing, staining, and immunophenotyping of AML and describes the principles of data analysis for AML classification and MRD testing.
通过多参数流式细胞术进行免疫表型分析是一种快速有效的技术，可同时评估和关联多个个体细胞特性，如大小和内部复杂性，以及一群细胞中的抗原表达。该方法用于在外周血（PB）和骨髓（BM）中快速鉴定原始细胞并对急性髓系白血病（AML）进行分类。这项技术不仅在初始诊断中有用，而且在通过微小残留病（MRD）检测监测和确定疾病预后方面也很有用。本章概述了AML标本处理、染色和免疫表型分析的程序，并描述了AML分类和MRD检测的数据分析原则。
