Zeng Zhihong, Konopleva Marina, Andreeff Michael
Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Methods Mol Biol. 2017;1633:75-86. doi: 10.1007/978-1-4939-7142-8_5.
Mass cytometry time-of-flight (CyTOF) empowers us to understand acute myeloid leukemia (AML) biology at the single-cell level. This technology, combined with advanced data-analysis methods, enables identification and characterization of the rare AML stem/progenitor cells that play key roles in drug resistance and AML relapse. Here we provide a protocol for AML sample preparation for CyTOF and keynotes emphasizing critical steps in and troubleshooting strategies for this procedure.
质谱流式细胞仪飞行时间分析技术（CyTOF）使我们能够在单细胞水平上了解急性髓系白血病（AML）的生物学特性。这项技术与先进的数据分析方法相结合，能够识别和表征在耐药性和AML复发中起关键作用的罕见AML干/祖细胞。在此，我们提供了一份用于CyTOF的AML样本制备方案，并重点介绍了该过程中的关键步骤及故障排除策略。
