Margiotta Azzurra, Progida Cinzia, Bakke Oddmund, Bucci Cecilia
University of Salento, Department of Biological and Environmental Sciences and Technologies.
Eur J Histochem. 2017 May 31;61(2):2783. doi: 10.4081/ejh.2017.2783.
Rab-interacting lysosomal protein (RILP) is a regulator of late stages of endocytosis. Recent work proved that depletion of RILP promotes migration of breast cancer cells in wound healing assay, whereas its overexpression influences re-arrangements of actin cytoskeleton. Here, we further characterized the role of RILP in cell migration by analyzing several aspects of this process. We showed that RILP is fundamental also for migration of lung cancer cells regulating cell velocity. RILP silencing did not affect Golgi apparatus nor microtubules reorientation during migration. However, both RILP over-expression and expression of its mutated form, RILP-C33, impair cell adhesion and spreading. In conclusion, our results demonstrate that RILP has important regulatory roles in cell motility affecting migration velocity but also in cell adhesion and cell spreading.
Rab 相互作用溶酶体蛋白（RILP）是内吞作用后期的一种调节因子。最近的研究表明，在伤口愈合实验中，RILP 的缺失会促进乳腺癌细胞的迁移，而其过表达则会影响肌动蛋白细胞骨架的重排。在此，我们通过分析这一过程的几个方面，进一步明确了 RILP 在细胞迁移中的作用。我们发现，RILP 对肺癌细胞的迁移也至关重要，它能够调节细胞速度。RILP 沉默在迁移过程中不影响高尔基体和微管的重新定向。然而，RILP 的过表达及其突变形式 RILP - C33 的表达都会损害细胞黏附和铺展。总之，我们的结果表明，RILP 在细胞运动中具有重要的调节作用，不仅影响迁移速度，还影响细胞黏附和细胞铺展。
