Sharma Shiv K, Leblanc Roger M
Department of Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, Florida 33146, USA.
Department of Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, Florida 33146, USA.
Anal Biochem. 2017 Oct 15;535:1-11. doi: 10.1016/j.ab.2017.07.019. Epub 2017 Jul 20.
Many industries are striving for the development of more reliable and robust β-galactosidase biosensors that exhibit high response rate, increased detection limit and enriched useful lifetime. In a newfangled technological atmosphere, a trivial advantage or disadvantage of the developed biosensor may escort to the survival and extinction of the industry. Several alternative strategies to immobilize β-galactosidase enzyme for their utilization in biosensors have been developed in recent years in the quest of maximum utility by controlling the defects seen in the previous biosensors. The overwhelming call for on-line measurement of different sample constituents has directed science and industry to search for best practical solutions and biosensors are witnessed as the best prospect. The main objective of this paper is to serve as a narrow footbridge by comparing the literary works on the β-galactosidase biosensors, critically analyze their use in the construction of best biosensor by showing the pros and cons of the predicted methods for the practical use of biosensors.
许多行业都在努力开发更可靠、更强大的β-半乳糖苷酶生物传感器，这些传感器具有高响应率、更低的检测限和更长的使用寿命。在全新的技术环境中，已开发的生物传感器的一个微小优势或劣势可能关乎该行业的生存与消亡。近年来，为了通过控制先前生物传感器中出现的缺陷来实现最大效用，人们已经开发出了几种将β-半乳糖苷酶固定化以用于生物传感器的替代策略。对不同样品成分进行在线测量的迫切需求促使科学界和工业界寻求最佳实用解决方案，而生物传感器被视为最有前景的方案。本文的主要目的是通过比较关于β-半乳糖苷酶生物传感器的文献，搭建一座狭窄的桥梁，通过展示预测方法在生物传感器实际应用中的优缺点，批判性地分析它们在构建最佳生物传感器中的应用。
