Jiang Liang, Peng Zhengwen, Guo Huizhen, Sun Jingchen, Sun Qiang, Xia Fei, Huang Chunlin, Xu Guowen, Xia Qingyou
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
Dev Comp Immunol. 2017 Dec;77:138-140. doi: 10.1016/j.dci.2017.07.020. Epub 2017 Jul 20.
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), a major pathogen of silkworms, causes serious economic losses in sericulture. The BmCPV genome contains 10 discrete dsRNA segments; among these, S1, S2, S3, S4, S6, and S7 encode virus structural proteins, whereas S5, S8, S9, and S10 encode nonstructural proteins. In an attempt to create an anti-BmCPV silkworm strain, we constructed transgenic RNAi vector pb-CNS for knockdown of S5, S8, S9, and S10, and pb-SNS targeting S1, S2, S4, S5, and S8. Transgenic silkworm line CNS and SNS were generated via microinjection of the practical diapause silkworm strain Furong. Following infection via the oral administration of a high dose of BmCPV, the mortality rates of the nontransgenic control, CNS, and SNS were 91%, 37%, and 41%, respectively. qPCR showed that the viral mRNA content in CNS and SNS was significantly lower than that in the nontransgenic line. The economic traits of CNS and SNS were not affected. These results suggest that the knockdown of multiple BmCPV genes significantly enhances the antiviral capacity of the silkworm.
家蚕细胞质型多角体病毒（BmCPV）是家蚕的主要病原体，给养蚕业造成严重经济损失。BmCPV基因组包含10个独立的双链RNA片段；其中，S1、S2、S3、S4、S6和S7编码病毒结构蛋白，而S5、S8、S9和S10编码非结构蛋白。为了培育抗BmCPV的家蚕品系，我们构建了用于敲低S5、S8、S9和S10的转基因RNA干扰载体pb-CNS，以及靶向S1、S2、S4、S5和S8的pb-SNS。通过显微注射实用滞育家蚕品系芙蓉，获得了转基因家蚕品系CNS和SNS。经口服高剂量BmCPV感染后，非转基因对照、CNS和SNS的死亡率分别为91%、37%和41%。定量PCR显示，CNS和SNS中的病毒mRNA含量显著低于非转基因品系。CNS和SNS的经济性状未受影响。这些结果表明，敲低多个BmCPV基因可显著增强家蚕的抗病毒能力。
