Jiang Liang, Wang Yumei, Guo Huizhen, Sun Qiang, Xie Enyu, Liuli Haoyu, Li Qing, Xia Qingyou
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Toxicol Res (Camb). 2020 Dec 18;9(6):845-853. doi: 10.1093/toxres/tfaa089. eCollection 2020 Dec.
Safety of transgenic silkworms must be evaluated before their commercial application. We assessed subacute toxicity using a 28-day feeding study in rats. Eighty rats were evenly allocated into four groups, with each group containing 10 male and 10 female rats. Rats of three groups were fed dried transgenic silkworm H19.9A pupae with overexpressed endogenous Bmhsp19.9, transgenic silkworm A4SOR pupae with overexpressed exogenous SOR, or normal silkworm pupae at a dose of 3.0 g/kg/day, respectively. The fourth group served as a normal feeding control. The body weight, feed consumption, hematology response variables, serum biochemical parameters, organ weights, gross necropsy, and histopathologic of animals were evaluated. No mortality, adverse effects, or major differences in the evaluated parameters were observed in the groups fed transgenic pupae in comparison with the control, suggesting that transgenic silkworms are toxicologically equivalent to normal silkworms and are safe for consumption in rats.
转基因家蚕在商业应用前必须进行安全性评估。我们通过对大鼠进行为期28天的喂养研究来评估亚急性毒性。80只大鼠被平均分为四组，每组包含10只雄性大鼠和10只雌性大鼠。三组大鼠分别以3.0 g/kg/天的剂量喂食过表达内源性Bmhsp19.9的转基因家蚕H19.9A蛹、过表达外源性SOR的转基因家蚕A4SOR蛹或正常家蚕蛹。第四组作为正常喂养对照。评估了动物的体重、饲料消耗量、血液学反应变量、血清生化参数、器官重量、大体尸检和组织病理学。与对照组相比，喂食转基因蛹的组未观察到死亡、不良反应或评估参数的主要差异，这表明转基因家蚕在毒理学上与正常家蚕相当，对大鼠食用是安全的。
