Sun Qiang, Jiang Liang, Guo Huizhen, Xia Fei, Wang Bingbing, Wang Yumei, Xia Qingyou, Zhao Ping
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
Dev Comp Immunol. 2018 Oct;87:188-192. doi: 10.1016/j.dci.2018.06.002. Epub 2018 Jun 23.
Bombyx mori bidensovirus (BmBDV) causes fatal flacherie disease leading to severe economic losses in sericultures. The BmDNV-Z genome contains two single-stranded DNA molecules, VD1 and VD2. For generating silkworm lines with antiviral properties, two transgenic RNA interference (RNAi) vectors were constructed. Open reading frames (ORFs) 1-4 of VD1 were knockdown by vector pb-BDV1 while ORF1a, ORF1b, and ORF3 of VD2 were knockdown by vector pb-BDV2. Transgenic silkworm lines BDV1-I and BDV2-I were generated via RNAi microinjection. Mortality rates of BDV1-I and BDV2-I were reduced by 45% and 39%, respectively, and quantitative PCR showed that VD1 and VD2 contents in BDV1-I and BDV2-I were significantly lower than in the non-transgenic line. However, economic traits showed no obvious differences. Thus, knockdown of multiple BmDNV-Z genes provides strong resistance to BDV1-I and BDV2-I lines, and these can be used in sericulture without hampering silk production.
家蚕双顺反子病毒（BmBDV）会引发致命的空头性软化病，给养蚕业造成严重经济损失。BmDNV-Z基因组包含两个单链DNA分子，即VD1和VD2。为了培育具有抗病毒特性的家蚕品系，构建了两种转基因RNA干扰（RNAi）载体。载体pb-BDV1可敲低VD1的开放阅读框（ORF）1-4，而载体pb-BDV2可敲低VD2的ORF1a、ORF1b和ORF3。通过RNAi显微注射获得了转基因家蚕品系BDV1-I和BDV2-I。BDV1-I和BDV2-I的死亡率分别降低了45%和39%，定量PCR显示BDV1-I和BDV2-I中VD1和VD2的含量显著低于非转基因品系。然而，经济性状没有明显差异。因此，敲低多个BmDNV-Z基因可使BDV1-I和BDV2-I品系获得强大的抗病毒能力，且这些品系可用于养蚕业而不影响蚕丝生产。
