研究猪骨骼肌中63个脂质代谢基因表达的遗传调控。

Investigating the genetic regulation of the expression of 63 lipid metabolism genes in the pig skeletal muscle.

作者信息

González-Prendes R, Quintanilla R, Amills M

机构信息

Department of Animal Genetics, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain.

Animal Breeding and Genetics Program, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Torre Marimon, Caldes de Montbui, 08140, Spain.

出版信息

Anim Genet. 2017 Oct;48(5):606-610. doi: 10.1111/age.12586. Epub 2017 Jul 24.

DOI:10.1111/age.12586

PMID:28737243

Abstract

A comprehensive and systematic view of the genetic regulation of lipid metabolism genes is still lacking in pigs. Herewith, we have investigated the genetic regulation of 63 porcine genes with crucial roles in the uptake, transport, synthesis and catabolism of lipids. With this aim, we have performed an expression QTL (eQTL) scan in 104 pigs with available genotypes for the Illumina Porcine SNP60 chip and microarray measurements of gene expression in the gluteus medius muscle. Analysis of the data with gemma software revealed 13 cis- and 18 trans-eQTL modulating the expression of 19 loci. Genes regulated by eQTL participated in a wide array of lipid metabolism pathways such as the β-oxidation of fatty acids, lipid biosynthesis and lipolysis, fatty acid activation and desaturation, lipoprotein uptake, apolipoprotein assembly and cholesterol trafficking. These data provide a first picture of the genetic regulation of loci involved in porcine lipid metabolism.

摘要

目前对猪脂质代谢基因的遗传调控仍缺乏全面系统的认识。在此，我们研究了63个在脂质摄取、转运、合成和分解代谢中起关键作用的猪基因的遗传调控。为此，我们对104头猪进行了表达数量性状基因座（eQTL）扫描，这些猪具有Illumina猪SNP60芯片的可用基因型，并对其臀中肌的基因表达进行了微阵列测量。使用gemma软件对数据进行分析，发现了13个顺式和18个反式eQTL，它们调控着19个基因座的表达。受eQTL调控的基因参与了广泛的脂质代谢途径，如脂肪酸的β-氧化、脂质生物合成和脂解、脂肪酸活化和去饱和、脂蛋白摄取、载脂蛋白组装和胆固醇运输。这些数据首次描绘了猪脂质代谢相关基因座的遗传调控情况。