M.V. Lomonosov Moscow State University, Chemistry Department, Leninskie Hills 1/3, GSP-1, 119991 Moscow, Russia.
University of Washington, Department of Bioengineering, Seattle, WA 98195, USA.
Talanta. 2017 Nov 1;174:206-213. doi: 10.1016/j.talanta.2017.06.001. Epub 2017 Jun 3.
Determination of chromophores of various classes-rosaniline (fuchsine), cyanidin-3-O-glucoside, tris(1,10-phenanthroline) iron(II), and phenol red - in their concentrated solutions near their solubility limits is performed with the optoacoustic technique for optically dense solutions; light-absorption coefficients of samples range from 0.5 to 500cm. The assessment of these substances in organo-aqueous and organic solvents is possible up to ca. 0.1molL. Characteristic stability and rate constants of the chelation of iron(II) with 1,10-phenanthroline are determined. It was found that turbidities up to 200 FTU and dynamic viscosities up to 20mPas do not affect the determination. The determination of total anthocyanins (as cyanidin-3-O-glucoside) in bilberry and sweet-cherry juices agrees with the reference spectrophotometric method and demonstrates the possibilities of the optoacoustic technique for the analysis of real samples without dilution and with almost no sample preparation.
在光学密度高的溶液中，使用光声技术测定其在接近溶解度极限的浓溶液中各种类别的生色团（副品红、矢车菊素-3-O-葡萄糖苷、三（1,10-菲咯啉）铁（II）和苯酚红）。对于有机-水相和有机溶剂中的这些物质，评估范围可达约 0.1mol/L。确定了铁（II）与 1,10-菲咯啉的螯合的特征稳定性和速率常数。结果发现，浊度高达 200 FTU 和动态粘度高达 20 mPas 不会影响测定。测定越桔和甜樱桃汁中的总花色苷（以矢车菊素-3-O-葡萄糖苷计）与参考分光光度法一致，并展示了光声技术在无需稀释和几乎无需样品制备的情况下分析实际样品的可能性。
