Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019, USA.
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Talanta. 2017 Nov 1;174:380-386. doi: 10.1016/j.talanta.2017.05.026. Epub 2017 May 11.
An enantioselective depletion study of praziquantel (PZQ) in perch muscle was done using a simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Two cyclic polypeptides and two cyclic saccharide chromatographic columns were evaluated. The new hydroxypropyl β-cyclodextrin (HP-RSP) superficially porous particle (SPP) column produced the optimum separation and was selected for the study. Deuterium labeled PZQ-d was used as the internal standard. The method was linear over concentration ranges of 5.00-5.00×10μgL (r≥0.99) for (-)-R-PZQ and (+)-S-PZQ. The average recoveries of R-PZQ and S-PZQ at three spiked levels of 5.00, 50.00 and 5.00×10μgkg ranged from 86.1% to 98.2%, and the intra-day and inter-day relative standard deviations were less than 5%. The decision limit (CCα) and detection capability (CCβ) of R-PZQ and S-PZQ in perch muscle matrices were all 1.0μgkg and 5.0μgkg, respectively. The method was successfully applied in monitoring the depletion of praziquantel enantiomers in perch muscle following oral administration. The elimination rate of R-PZQ and S-PZQ in perch muscle tissue is equivalent.
采用简单、灵敏、特异的液质联用法对鲈鱼肌肉中的吡喹酮（PZQ）进行了对映体选择性耗竭研究。评估了两种环多肽和两种环糖色谱柱。新的羟丙基-β-环糊精（HP-RSP）表面多孔颗粒（SPP）柱产生了最佳分离效果，并被选为研究用柱。氘标记的 PZQ-d 被用作内标。该方法在 (-)-R-PZQ 和 (+)-S-PZQ 的浓度范围为 5.00-5.00×10μgL（r≥0.99）时呈线性。R-PZQ 和 S-PZQ 在三个添加水平（5.00、50.00 和 5.00×10μgkg）的平均回收率在 86.1%至 98.2%之间，日内和日间相对标准偏差均小于 5%。R-PZQ 和 S-PZQ 在鲈鱼肌肉基质中的决策限（CCα）和检测能力（CCβ）均为 1.0μgkg 和 5.0μgkg。该方法成功应用于监测口服给药后鲈鱼肌肉中吡喹酮对映体的耗竭情况。R-PZQ 和 S-PZQ 在鲈鱼肌肉组织中的消除率相当。
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