Baralla Elena, Varoni Maria Vittoria, Nieddu Maria, Demontis Maria Piera, Merella Paolo, Burreddu Caterina, Garippa Giovanni, Boatto Gianpiero
Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy.
Department of Chemistry and Pharmacy, University of Sassari, 07100 Sassari, Italy.
Animals (Basel). 2020 Mar 21;10(3):528. doi: 10.3390/ani10030528.
Praziquantel (PZQ) is an anthelmintic drug used in humans and animals against Platyhelminthes and in aquaculture in the Far East. Medicated feed is one of the most convenient forms of oral administration of drugs in aquaculture because it allows to treat a large population of fish in an easy way. However, this treatment may lead to residues in fish intended for human consumption. In this study, a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed in order to verify the presence of PZQ in samples of after oral administration of feed treated with PZQ. The method was validated according to international guidelines. It showed good recoveries, selectivity and sensitivity (LOD and LOQ were 3.0 and 9.3 ng/g, respectively), with precision and matrix effect values ≤ 15%. This method could also be applied to determine PZQ residue in other fish species and thus to evaluate the appropriate withdrawal time in treated fish intended for human consumption.
吡喹酮（PZQ）是一种用于人和动物对抗扁形虫的驱虫药，在远东地区的水产养殖中也有使用。药饵是水产养殖中口服给药最方便的形式之一，因为它能以简便的方式治疗大量鱼类。然而，这种治疗方式可能会导致供人类食用的鱼类中出现药物残留。在本研究中，开发了一种液相色谱-串联质谱（LC-MS/MS）方法，以验证在投喂经PZQ处理的饲料后，鱼样本中PZQ的存在情况。该方法根据国际指南进行了验证。它显示出良好的回收率、选择性和灵敏度（检测限和定量限分别为3.0和9.3 ng/g），精密度和基质效应值≤15%。该方法还可用于测定其他鱼类品种中的PZQ残留，从而评估供人类食用的经处理鱼类的适当休药期。
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