Schönherr-Hellec S, Klein G, Delannoy J, Ferraris L, Friedel I, Rozé J C, Butel M J, Aires J
EA 4065, Faculty of Pharmacy, Paris Descartes University, Paris, France.
Department of Neonatal Medicine, Nantes University Hospital, Nantes, France.
Anaerobe. 2017 Dec;48:76-82. doi: 10.1016/j.anaerobe.2017.07.002. Epub 2017 Jul 21.
"Clostridium neonatale" was recently described as a new species within the Cluster I of the Clostridium genus sensu stricto. In this study, we characterized "C. neonatale" isolates (n = 42) and compared their phenotypic properties with those of Clostridium butyricum (n = 26), a close related species. Strains isolated from fecal samples of healthy neonates were tested for different phenotypic characteristics. Compared to C. butyricum, "C. neonatale" showed a significant higher surface hydrophobicity (p = 0.0047), exopolysaccharide production (p = 0.0069), aero-tolerance (p = 0.0222) and viability at 30 °C (p = 0.0006). A lower swimming ability (p = 0.0146) and tolerance against bile (0.3%) (p = 0.0494), acid (pH 4.5) (p < 0.0001), osmolarity (NaCl 5%, p = 0.0188) and temperature at 50 °C (p = 0.0013) characterized "C. neonatale" strains. Our results showed that "C. neonatale" behaves very differently from C. butyricum and suggests specific responses to environmental changes. Besides it is the first study on clinical isolates for these two anaerobic members of the newborns' gut microbiota and broadens our knowledge about their phenotypic traits.
“新生儿梭菌”最近被描述为严格意义上梭菌属第一群中的一个新物种。在本研究中，我们对“新生儿梭菌”分离株（n = 42）进行了特性分析，并将其表型特性与密切相关的丁酸梭菌（n = 26）进行了比较。对从健康新生儿粪便样本中分离出的菌株进行了不同表型特征的检测。与丁酸梭菌相比，“新生儿梭菌”表现出显著更高的表面疏水性（p = 0.0047）、胞外多糖产生量（p = 0.0069）、耐氧性（p = 0.0222）以及在30°C时的生存能力（p = 0.0006）。“新生儿梭菌”菌株的游动能力较低（p = 0.0146），对胆汁（0.3%）（p = 0.0494）、酸（pH 4.5）（p < 0.0001）、渗透压（NaCl 5%，p = 0.0188）和50°C温度（p = 0.0013）的耐受性较低。我们的结果表明，“新生儿梭菌”的行为与丁酸梭菌有很大不同，并表明其对环境变化有特定反应。此外，这是对新生儿肠道微生物群这两种厌氧成员临床分离株的首次研究，拓宽了我们对其表型特征的认识。
