Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198, Assistance Publique - Hôpitaux de Marseille (AP-HM), Microbes, Evolution, Phylogeny and Infection (MEΦI), Institut Hospitalo-Universitaire (IHU) - Méditerranée Infection, Marseille, France.
Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198, Assistance Publique - Hôpitaux de Marseille (AP-HM), Vecteurs - Infections TROpicales et MÉditerrannéennes (VITROME), Service de Santé des Armées, Institut Hospitalo-Universitaire (IHU) - Méditerranée Infection, Marseille, France.
Sci Rep. 2019 Feb 14;9(1):2077. doi: 10.1038/s41598-019-38773-7.
The association between Clostridium species identification from stool samples in preterm neonates and the occurrence of necrotizing enterocolitis has been increasingly reported. To confirm the specific impact of Clostridium butyricum in this pathology, selective culture procedure was used for Clostridia isolation. Whole-genome analysis was employed to investigate genomic relationships between isolates. Stool samples from present study, as well as from previously investigated cases, were implicated including 88 from preterm neonates with necrotizing enterocolitis and 71 from matched controls. Quantitative real-time polymerase chain reaction was performed to evaluate the presence of C. butyricum from stools of new cases. Clostridium species prevalence isolated by culture was compared between patients with necrotizing enterocolitis and controls. By combining results of both culture and quantitative polymerase chain reaction methods, C. butyricum was significantly more frequent in stool samples from preterm neonates with necrotizing enterocolitis than in controls. Whole-genome analysis of 81 genomes including 58 neonates' isolates revealed that cases were clustered depending on geographical origin of isolation. Controls isolates presented genomic relations with that of patients suggesting a mechanism of asymptomatic carriage. Overall, this suggests an epidemiology comparable to that observed in Clostridium difficile colitis in adults.
越来越多的研究报道称，从早产儿粪便样本中鉴定出的梭状芽孢杆菌与坏死性小肠结肠炎的发生有关。为了证实丁酸梭菌在这一病理过程中的具体影响，采用选择性培养程序分离梭状芽孢杆菌。对分离株进行全基因组分析，以研究其基因组之间的关系。本研究的粪便样本，以及以前调查过的病例，包括 88 例患有坏死性小肠结肠炎的早产儿和 71 例匹配对照。采用定量实时聚合酶链反应（PCR）评估新病例粪便中丁酸梭菌的存在。比较坏死性小肠结肠炎患者和对照组粪便中培养分离的梭菌的流行情况。通过结合培养和定量聚合酶链反应方法的结果，患有坏死性小肠结肠炎的早产儿粪便中丁酸梭菌的频率明显高于对照组。对包括 58 例新生儿分离株在内的 81 个基因组的全基因组分析显示，病例根据分离的地理位置聚类。对照组的分离株与患者的分离株具有基因组关系，提示存在无症状携带的机制。总的来说，这表明其流行病学与成人中观察到的艰难梭菌结肠炎相似。
