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从街头售卖食品及相关环境中分离出的微生物的抗微生物耐药性及分型

Antimicrobial resistance and typing of isolated from street vended foods and associated environment.

作者信息

Shagufta Bi, Sivakumar M, Kumar Surender, Agarwal Rajesh Kumar, Bhilegaonkar Kiran Narayan, Kumar Ashok, Dubal Zunjar Baburao

机构信息

Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, U.P. 243 122 India.

出版信息

J Food Sci Technol. 2017 Jul;54(8):2532-2539. doi: 10.1007/s13197-017-2698-1. Epub 2017 May 29.

Abstract

The present study was carried out to find out the occurrence and types of present in street vended foods and associated environment, and their resistance pattern against various antibiotics. About 1075 street vended food and associated environment samples were processed for isolation and confirmation of different spp. by targeting gene specific A gene and serotype specific I, and genes by PCR. Selected isolates were screened for antibiotic resistance by using Baeur-Kirby disk diffusion test. Out of 1075 samples, only 31 (2.88%) isolates could be amplified the A gene of which 19 could be recovered from meat vendors; 8 from egg vendors while remaining 4 from milk vendors. Though, majority of recovered from raw foods the ready-to-eat food like chicken gravy and rasmalai also showed its presence which pose a serious public health threat. Overall, 19, 6 and 1 isolates of Typhimurium, Enteritidis and Typhi could be detected by PCR while remaining 5 isolates could not be amplified suggesting other type of Selected isolates were completely resistance to Oxacillin (100%) followed by Cefoxitin (30.43%) and Ampicillin (26.10%). Thus, it is observed that the street vended foods of animal origin and associated environment play an important role in transmission of food borne pathogens including .

摘要

本研究旨在查明街头售卖食品及其相关环境中存在的[病原体名称]的种类和类型,以及它们对各种抗生素的耐药模式。通过针对基因特异性A基因以及通过PCR检测血清型特异性I、[血清型名称]和[血清型名称]基因,对约1075份街头售卖食品及其相关环境样本进行了处理,以分离和鉴定不同的[病原体名称]菌株。使用鲍氏-柯氏纸片扩散法对选定的[病原体名称]分离株进行抗生素耐药性筛选。在1075份样本中,只有31份(2.88%)分离株能够扩增出A基因,其中19份从肉类摊贩处分离得到;8份从蛋类摊贩处分离得到,其余4份从奶类摊贩处分离得到。虽然,从生食中分离出的大多数[病原体名称],像鸡肉肉汁和印度奶酪等即食食品中也检测到了该病原体,这对公众健康构成了严重威胁。总体而言,通过PCR检测到19株鼠伤寒沙门氏菌、6株肠炎沙门氏菌和1株伤寒沙门氏菌,而其余5株分离株未能扩增出来,提示存在其他类型的[病原体名称]。选定的[病原体名称]分离株对苯唑西林完全耐药(100%),其次是头孢西丁(30.43%)和氨苄西林(26.10%)。因此,可以观察到动物源性街头售卖食品及其相关环境在包括[病原体名称]在内的食源性病原体传播中起着重要作用。

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