Bajer Lukas, Kverka Miloslav, Kostovcik Martin, Macinga Peter, Dvorak Jiri, Stehlikova Zuzana, Brezina Jan, Wohl Pavel, Spicak Julius, Drastich Pavel
Lukas Bajer, Peter Macinga, Jan Brezina, Pavel Wohl, Julius Spicak, Pavel Drastich, Department of Gastroenterology and Hepatology, Institute for Clinical and Experimental Medicine, 14021 Prague, Czech Republic.
World J Gastroenterol. 2017 Jul 7;23(25):4548-4558. doi: 10.3748/wjg.v23.i25.4548.
To characterize the gut bacterial microbiota of patients with primary sclerosing cholangitis (PSC) and ulcerative colitis (UC).
Stool samples were collected and relevant clinical data obtained from 106 study participants, 43 PSC patients with ( = 32) or without ( = 11) concomitant inflammatory bowel disease, 32 UC patients, and 31 healthy controls. The V3 and V4 regions of the 16S ribosomal RNA gene were sequenced on Illumina MiSeq platform to cover low taxonomic levels. Data were further processed in QIIME employing MaAsLin and LEfSe tools for analysis of the output data.
Microbial profiles in both PSC and UC were characterized by low bacterial diversity and significant change in global microbial composition. , , , , and three other genera were markedly overrepresented in PSC regardless of concomitant inflammatory bowel disease (IBD). , and were tracked to the species level to identify , , , and along with and . PSC was further characterized by decreased abundance of and . Decrease in genus was linked to presence of colonic inflammation regardless of IBD phenotype. , and were decreased in UC along with genus . Low levels of serum albumin were significantly correlated with enrichment of order Actinomycetales.
PSC is associated with specific gut microbes independently of concomitant IBD and several bacterial taxa clearly distinguish IBD phenotypes (PSC-IBD and UC).
对原发性硬化性胆管炎（PSC）和溃疡性结肠炎（UC）患者的肠道细菌微生物群进行特征分析。
收集了106名研究参与者的粪便样本并获取了相关临床数据，其中包括43例PSC患者，其中32例伴有炎性肠病，11例不伴有炎性肠病，32例UC患者以及31名健康对照者。在Illumina MiSeq平台上对16S核糖体RNA基因的V3和V4区域进行测序，以涵盖低分类水平。数据在QIIME中进一步处理，采用MaAsLin和LEfSe工具对输出数据进行分析。
PSC和UC中的微生物谱均以细菌多样性低和整体微生物组成的显著变化为特征。无论是否伴有炎性肠病（IBD），在PSC中，[具体菌属1]、[具体菌属2]、[具体菌属3]、[具体菌属4]、[具体菌属5]和其他三个菌属的含量均明显过高。[具体菌属1]、[具体菌属2]和[具体菌属3]追踪到种水平，分别鉴定为[具体菌种1]、[具体菌种2]、[具体菌种3]、[具体菌种4]以及[具体菌种5]和[具体菌种6]。PSC的进一步特征是[具体菌属6]和[具体菌属7]的丰度降低。无论IBD表型如何，[具体菌属8]的减少与结肠炎症的存在有关。在UC中，[具体菌属9]、[具体菌属10]和[具体菌属11]以及[具体菌属8]均减少。血清白蛋白水平低与放线菌目丰度增加显著相关。
PSC与特定的肠道微生物有关，独立于伴随的IBD，并且几种细菌分类群可明确区分IBD表型（PSC-IBD和UC）。
