Institute of Digestive Diseases, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Department of Bioinformatics, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
World J Gastroenterol. 2019 Jul 7;25(25):3242-3255. doi: 10.3748/wjg.v25.i25.3242.
Ulcerative colitis (UC) is considered to be closely associated with alteration of intestinal microorganisms. According to the traditional Chinese medicine (TCM) theory, UC can be divided into two disease syndromes called Pi-Xu-Shi-Yun (PXSY) and Da-Chang-Shi-Re (DCSR). The relationships among gut microbiota, TCM syndromes, and UC pathogenesis have not been well investigated.
To investigate the role of gut microbiota in UC and the distinction of microbiota dysbiosis between PXSY and DCSR syndromes.
From May 2015 to February 2016, UC patients presenting to LongHua Hospital who met the established inclusion and exclusion criteria were enrolled in this retrospective study. Fresh stool specimens of UC patients with PXSY or DCSR were collected. The feces of the control group came from the health examination population of Longhua Hospital. The composition of gut bacterial communities in stool samples was determined by the pyrosequencing of 16S ribosomal RNA. The high-throughput sequencing reads were processed with QIIME, and biological functions were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.
The composition of gut bacterial communities in 93 stool samples (30 healthy controls, 32 patients with PXSY syndrome, and 31 patients with DCSR syndrome) was determined by the pyrosequencing of 16S ribosomal RNA. Beta diversity showed that the composition of the microbiota was different among the three groups. At the family level, Porphyromonadaceae, Rikeneliaceae, and Lachnospiraceae significantly decreased while Enterococcus, Streptococcus, and other potential pathogens significantly increased in UC patients compared to healthy subjects. At the genus level, , , and decreased while showed increased abundance in UC compared to healthy controls. Five differential taxa were identified between PXSY and DCSR syndromes. At the genus level, a significantly increased abundance of was observed in DCSR patients, while increased in PXSY patients. The differential functional pathways of the gut microbiome between the PXSY and DCSR groups mainly included lipid metabolism, immunity, and the metabolism of polypeptides.
Our study suggests that the gut microbiota contributes to the distinction between the two TCM syndromes of UC.
溃疡性结肠炎（UC）被认为与肠道微生物的改变密切相关。根据中医理论，UC 可分为脾失健运（PXSY）和大肠湿热（DCSR）两种证候。肠道微生物群、中医证候与 UC 发病机制之间的关系尚未得到很好的研究。
探讨肠道微生物群在 UC 中的作用及 PXSY 和 DCSR 证候之间肠道微生物失调的区别。
本研究为回顾性研究，于 2015 年 5 月至 2016 年 2 月期间招募符合既定纳入和排除标准的龙华医院就诊的 UC 患者。收集 UC 患者 PXSY 或 DCSR 粪便标本。对照组粪便来自龙华医院体检人群。采用 16S 核糖体 RNA 焦磷酸测序法测定粪便样本中肠道细菌群落的组成。高通量测序读段用 QIIME 处理，用未观察状态重建的群落系统发育分析预测生物功能。
采用 16S 核糖体 RNA 焦磷酸测序法测定了 93 份粪便样本（30 名健康对照、32 名 PXSY 综合征患者和 31 名 DCSR 综合征患者）的肠道细菌群落组成。β多样性分析显示，三组之间的微生物组成不同。在科水平上，与健康对照组相比，UC 患者的拟杆菌科、理研菌科和lachnospiraceae 显著减少，而肠球菌、链球菌和其他潜在病原体显著增加。在属水平上， ， ， 和 减少，而 UC 对照健康对照中丰度增加。在 PXSY 和 DCSR 综合征之间鉴定出 5 个差异分类群。在属水平上，DCSR 患者的 丰度显著增加，而 PXSY 患者的 增加。PXSY 和 DCSR 组肠道微生物群的差异功能途径主要包括脂质代谢、免疫和多肽代谢。
本研究表明，肠道微生物群有助于区分 UC 的两种中医证候。
