Pechar R, Killer J, Švejstil R, Salmonová H, Geigerová M, Bunešová V, Rada V, Benada O
Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Kamýcká 129, Prague 6 - Suchdol, 165 00, Czech Republic.
Institute of Animal Physiology and Genetics v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4 - Krč, 142 20, Czech Republic.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2403-2411. doi: 10.1099/ijsem.0.001972. Epub 2017 Jul 21.
Bacteria with potential probiotic applications are not yet sufficiently explored, even for animals with economic importance. Therefore, we decided to isolate and identify representatives of the family Bifidobacteriaceae, which inhabit the crop of laying hens. During the study, a fructose-6-phosphate phosphoketolase-positive strain, RP51T, with a regular/slightly irregular and sometimes an S-shaped slightly curved rod-like shape, was isolated from the crop of a 13 -month-old Hisex Brown hybrid laying hen. The best growth of the Gram-stain-positive bacterium, which was isolated using Bifidobacterium-selective mTPY agar, was found out to be under strictly anaerobic conditions, however an ability to grow under microaerophilic and aerobic conditions was also observed. Sequencing of the almost complete 16S rRNA gene (1444 bp) showed Alloscardovia omnicolens CCUG 31649T and Bombiscardovia coagulans BLAPIII/AGVT to be the most closely related species with similarities of 93.4 and 93.1 %, respectively. Lower sequence similarities were determined with other scardovial genera and other representatives of the genus Bifidobacterium. Taxonomic relationships with A. omnicolens and other members of the family Bifidobacteriaceaewere also demonstrated, based on the sequences of dnaK, fusA, hsp60 and rplB gene fragments. Low sequence similarities of phylogenetic markers to related scardovial genera and bifidobacteria along with unique features of the bacterial strain investigated within the family Bifidobacteriaceae(including the lowest DNA G+C value (44.3 mol%), a unique spectrum of cellular fatty acids and polar lipids, cellular morphology, the wide temperature range for growth (15-49 °C) and habitat) clearly indicate that strain RP51T is a representative of a novel genus within the family Bifidobacteriaceae for which the name Galliscardovia ingluviei gen. nov., sp. nov. (RP51T=DSM 100235T=LMG 28778T=CCM 8606T) is proposed.
具有潜在益生菌应用价值的细菌尚未得到充分研究，即使是对于具有经济重要性的动物也是如此。因此，我们决定分离并鉴定栖息于蛋鸡嗉囊中的双歧杆菌科代表菌株。在这项研究中，从一只13月龄的海赛克斯褐壳蛋鸡杂交种的嗉囊中分离出了一株磷酸果糖-6-磷酸磷酸酮醇酶阳性菌株RP51T，其形态规则/略不规则，有时呈S形略弯曲的杆状。使用双歧杆菌选择性mTPY琼脂分离出的这株革兰氏阳性菌，在严格厌氧条件下生长最佳，但也观察到其在微需氧和需氧条件下的生长能力。对几乎完整的16S rRNA基因（1444bp）进行测序表明，全栖异斯卡多维亚菌CCUG 31649T和凝结双歧斯卡多维亚菌BLAPIII/AGVT是最密切相关的物种，相似性分别为93.4%和93.1%。与其他斯卡多维亚属和双歧杆菌属的其他代表菌株的序列相似性较低。基于dnaK、fusA、hsp60和rplB基因片段的序列，也证明了该菌株与全栖异斯卡多维亚菌及双歧杆菌科其他成员的分类关系。系统发育标记与相关斯卡多维亚属和双歧杆菌的序列相似性较低，以及在双歧杆菌科内所研究的细菌菌株的独特特征（包括最低的DNA G+C值（44.3mol%）、独特的细胞脂肪酸和极性脂质谱、细胞形态、广泛的生长温度范围（15-49°C）和栖息地）清楚地表明，菌株RP51T是双歧杆菌科内一个新属的代表，为此提出新属名Galliscardovia ingluviei gen. nov., sp. nov.（RP51T = DSM 100235T = LMG 28778T = CCM 8606T）。
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