McKeown Dean A, Stevens Kim, Peters Akira F, Bond Peter, Harper Glenn M, Brownlee Colin, Brown Murray T, Schroeder Declan C
Viral Ecology, Marine Biological, Association, Citadel Hill, Plymouth, UK.
School of Biological and Marine Sciences, University of Plymouth, Drake Circus, Plymouth, UK.
ISME J. 2017 Dec;11(12):2869-2873. doi: 10.1038/ismej.2017.130. Epub 2017 Jul 25.
Phaeoviruses are latent double-stranded DNA viruses that insert their genomes into those of their brown algal (Phaeophyceae) hosts. So far these viruses are known only from members of the Ectocarpales, which are small and short-lived macroalgae. Here we report molecular and morphological evidence for a new Phaeovirus cluster, referred to as sub-group C, infecting kelps (Laminariales) of the genera Laminaria and Saccharina, which are ecologically and commercially important seaweeds. Epifluorescence and TEM observations indicate that the Laminaria digitata Virus (LdigV), the type species of sub-group C, targets the host nucleus for its genome replication, followed by gradual degradation of the chloroplast and assembly of virions in the cytoplasm of both vegetative and reproductive cells. This study is the first to describe phaeoviruses in kelp. In the field, these viruses infected two thirds of their host populations; however, their biological impact remains unknown.
褐藻病毒是潜伏性双链DNA病毒，可将其基因组插入褐藻（褐藻纲）宿主的基因组中。到目前为止，这些病毒仅在网地藻目中被发现，网地藻目是小型且寿命较短的大型藻类。在此，我们报告了一种新的褐藻病毒簇（称为C亚群）的分子和形态学证据，该病毒簇感染海带属（海带目）的海带和糖海带，这些藻类在生态和商业上都很重要。落射荧光和透射电镜观察表明，C亚群的模式种——掌状海带病毒（LdigV），其基因组在宿主细胞核中进行复制，随后叶绿体逐渐降解，病毒粒子在营养细胞和生殖细胞的细胞质中组装。本研究首次描述了海带中的褐藻病毒。在野外，这些病毒感染了三分之二的宿主种群；然而，它们的生物学影响尚不清楚。
