Department of Interventional Radiology, Huizhou First Hospital, Huizhou City, Guangdong Province, China.
Eur Rev Med Pharmacol Sci. 2017 Jul;21(13):3054-3060.
Liver cancer is one of the common gastrointestinal cancers. This study was designed to investigate the effect of the cytokine-induced apoptosis inhibitor 1 (CIAPIN1) on hepatocellular carcinoma cell proliferation and invasion.
To establish a low and high expression of CIAPIN1 in hepatoma cell lines, pGPU6/GFP/Neo and CIAPIN1 siRNA vectors were constructed. The growth curve of liver cancer cells with a low and high expression of CIAPIN1 was measured by MTT assay and colony formation in soft. The effect of overexpression and inhibition of CIAPIN1 on the expressions of cell cycle proteins Cyclin D1, CDK2, CDK4, and Cyclin E were detected by western blot.
As compared with the low expression group, the cells in CIAPIN1 high expression group showed a significant decrease in proliferation (p < 0.05). In addition, the colony-forming ability of cells with high expression of CIAPIN1 was decreased significantly (p < 0.01). Furthermore, the expressions of Cyclin D1 CDK2, CDK4, and Cyclin E in high expression group were significantly increased (p < 0.01).
CIAPIN1 played an important role in the proliferation of liver cancer cells through increasing the expressions of cell cycle related proteins Cyclin D1, CDK2, CDK4, and Cyclin E.
肝癌是常见的胃肠道癌症之一。本研究旨在探讨细胞因子诱导凋亡抑制因子 1（CIAPIN1）对肝癌细胞增殖和侵袭的影响。
构建了 pGPU6/GFP/Neo 和 CIAPIN1 siRNA 载体，以建立低表达和高表达 CIAPIN1 的肝癌细胞系。通过 MTT 法和软琼脂集落形成实验测量低表达和高表达 CIAPIN1 的肝癌细胞的生长曲线。通过 Western blot 检测过表达和抑制 CIAPIN1 对细胞周期蛋白 Cyclin D1、CDK2、CDK4 和 Cyclin E 表达的影响。
与低表达组相比，CIAPIN1 高表达组细胞增殖明显减少（p < 0.05）。此外，CIAPIN1 高表达组细胞的集落形成能力明显下降（p < 0.01）。进一步的，高表达组细胞中 Cyclin D1、CDK2、CDK4 和 Cyclin E 的表达明显增加（p < 0.01）。
CIAPIN1 通过增加细胞周期相关蛋白 Cyclin D1、CDK2、CDK4 和 Cyclin E 的表达，在肝癌细胞的增殖中发挥重要作用。
