Kienzle Clara, Poulson Rebecca L, Ruder Mark G, Stallknecht David E
1 Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, Department of Population Health, The University of Georgia, 589 D. W. Brooks Drive, Athens, Georgia 30602, USA.
J Wildl Dis. 2017 Oct;53(4):843-849. doi: 10.7589/2017-02-040. Epub 2017 Jul 25.
Hemorrhagic disease in North America is caused by multiple serotypes of epizootic hemorrhagic disease virus (EHDV) and bluetongue virus (BTV). Diagnostic tests for detection of EHDV and BTV include virus isolation (VI), reverse transcriptase (RT)-PCR, and real-time RT-PCR (rRT-PCR). Our objective was to compare the diagnostic capabilities of three rRT-PCR protocols for detection of EHDV and BTV from naturally infected white-tailed deer (Odocoileus virginianus). We compared the effectiveness of these assays to traditional viral detection methods (e.g., VI) for historic and current clinical cases. Because of the variable nature of tissue collection and storage before diagnostic testing, an evaluation of viral persistence on multiple freeze-thaw events was also conducted. Two of the rRT-PCR assays provided for reliable detection of EHDV and BTV from 100% of clinically affected and VI-confirmed infected animals. Additionally, no significant change in viral titer was observed on multiple freeze-thaw events.
北美洲的出血性疾病是由多种血清型的流行性出血性疾病病毒（EHDV）和蓝舌病病毒（BTV）引起的。用于检测EHDV和BTV的诊断测试包括病毒分离（VI）、逆转录酶（RT）-PCR和实时RT-PCR（rRT-PCR）。我们的目标是比较三种rRT-PCR方案从自然感染的白尾鹿（弗吉尼亚鹿）中检测EHDV和BTV的诊断能力。我们将这些检测方法的有效性与传统病毒检测方法（如病毒分离）用于历史和当前临床病例的情况进行了比较。由于诊断测试前组织采集和储存的性质各不相同，还对多次冻融事件中病毒的持久性进行了评估。两种rRT-PCR检测方法能够从100%临床感染且经病毒分离确认感染的动物中可靠地检测出EHDV和BTV。此外，多次冻融事件未观察到病毒滴度有显著变化。
