Qi Xuefeng, Xu Jiamin, Wang Zugui, Wang Xueping, Wang Jingyu
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
Res Vet Sci. 2017 Oct;114:395-400. doi: 10.1016/j.rvsc.2017.07.015. Epub 2017 Jul 17.
Although extensive efforts have been made to understand adenovirus infection in human cells, little is known for egg drop syndrome virus (EDSV) infection in the avian-derived cells. In this study, the effects of EDSV infection as well as the possible role hexon protein, the main building block of the EDSV capsid, on apoptosis induction in duck embryo fibroblast (DEF) cells was examined. Flow cytometry analysis and TUNEL assay revealed that EDSV infection induced significant apoptosis in DEF cells compared with mock infected cells. Interestingly, the increase of the apoptosis rate detected in EDSV infected DEF cells were accompanied by an increased virus load in cells in a time-dependent manner. Furthermore, a time-dependent decrease in hexon protein expression levels in hexon transfected DEF cells in parallel with a gradual decrease in TUNEL-labeling cells was also observed in the current study. In addition, caspase activity detection and western blot analysis indicates that either EDSV infection or EDSV hexon transfection both induced apoptosis of DEF cells via activating both the exogenous and the mitochondrial pathway.
尽管人们已经付出了巨大努力来了解腺病毒在人类细胞中的感染情况，但对于禽源细胞中禽腺病毒-12型（EDSV）感染的了解却很少。在本研究中，检测了EDSV感染以及EDSV衣壳的主要组成部分六邻体蛋白对鸭胚成纤维细胞（DEF）凋亡诱导的影响。流式细胞术分析和TUNEL检测显示，与模拟感染细胞相比，EDSV感染可诱导DEF细胞发生显著凋亡。有趣的是，在EDSV感染的DEF细胞中检测到的凋亡率增加与细胞中病毒载量的增加呈时间依赖性。此外，在本研究中还观察到，六邻体转染的DEF细胞中六邻体蛋白表达水平随时间下降，同时TUNEL标记细胞也逐渐减少。此外，半胱天冬酶活性检测和蛋白质免疫印迹分析表明，EDSV感染或EDSV六邻体转染均可通过激活外源性和线粒体途径诱导DEF细胞凋亡。
