College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Poult Sci. 2019 Jan 1;98(1):404-412. doi: 10.3382/ps/pey318.
Egg drop syndrome virus (EDSV), a member of the family Adenoviridae and an economically important pathogen with a broad host range, leads to markedly decreased egg production. However, the molecular mechanism underlying the host-EDSV interaction remains unclear. Here, we performed high-throughput RNA sequencing (RNA-Seq) to study the dynamic changes in host gene expression at 6, 12, and 24 hours post-infection in duck embryo fibroblasts (DEFs) infected with EDSV. Atotal of 441 differentially expressed genes (DEGs) were identified after EDSV infection. Gene Ontology category and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that these DEGs were associated with multiple biological functions, including signal transduction, host immunity, virus infection, cell apoptosis, cell proliferation, and pathogenicity-related and metabolic process signaling pathways. We screened and identified 12 DEGs for further examination by using qRT-PCR. The qRT-PCR and RNA-Seq results were highly consistent. This study analyzed viral infection and host immunity induced by EDSV infection from a novel perspective, and the results provide valuable information regarding the mechanisms underlying host-EDSV interactions, which will prove useful for the future development of antiviral drugs or vaccines for poultry, thus benefiting the entire poultry industry.
蛋鸡产蛋下降综合征病毒（EDSV）属于腺病毒科，是一种具有广泛宿主范围的重要经济病原，可导致明显的产蛋量下降。然而，宿主与 EDSV 相互作用的分子机制尚不清楚。在这里，我们进行了高通量 RNA 测序（RNA-Seq），以研究感染 EDSV 后 6、12 和 24 小时鸭胚成纤维细胞（DEF）中宿主基因表达的动态变化。在 EDSV 感染后，共鉴定出 441 个差异表达基因（DEG）。基因本体论（GO）类别和京都基因与基因组百科全书（KEGG）途径富集分析表明，这些 DEG 与多种生物学功能相关，包括信号转导、宿主免疫、病毒感染、细胞凋亡、细胞增殖以及与致病性和代谢过程相关的信号通路。我们使用 qRT-PCR 进一步筛选和鉴定了 12 个 DEG。qRT-PCR 和 RNA-Seq 结果高度一致。本研究从新的角度分析了 EDSV 感染引起的病毒感染和宿主免疫，研究结果为宿主与 EDSV 相互作用的机制提供了有价值的信息，这将有助于未来开发针对家禽的抗病毒药物或疫苗，从而使整个家禽行业受益。
