Rivas-Aravena Andrea, Muñoz Patricio, Jorquera Patricia, Diaz Alvaro, Reinoso Claudia, González-Catrilelbún Sebastián, Sandino Ana María
Comisión Chilena de Energía Nuclear, Departamento de Aplicaciones Nucleares, Laboratorio de Radiobiología Celular y Molecular. Nueva Bilbao 12501, Las Condes, Santiago, Chile; Universidad San Sebastián, Facultad de Ciencias, Lota 2465, Providencia, Santiago, Chile.
Universidad de Santiago de Chile, Centro de Biotecnología Acuícola, Laboratorio de Virología,Av. Bernardo O'Higgins 3303, Estación Central, Santiago, Chile.
Virus Res. 2017 Aug 15;240:121-129. doi: 10.1016/j.virusres.2017.07.014. Epub 2017 Jul 23.
The infectious pancreatic necrosis virus (IPNV) is a salmonid pathogen that causes significant economic losses to the aquaculture industry. IPNV is a non-enveloped virus containing two uncapped and non-polyadenylated double strand RNA genomic segments, RNA-A and RNA-B. The viral protein Vpg is covalently attached to the 5' end of both segments. There is little knowledge about its viral cycle, particularly about the translation of the RNAs. Through experiments using mono and bicistronic reporters, in this work we show that the 120-nucleotide-long 5'-UTR of RNA-A contains an internal ribosome entry site (IRES) that functions efficiently both in vitro and in salmon cells. IRES activity is strongly dependent on temperature. Also, the IRES structure is confined to the 5'UTR and is not affected by the viral coding sequence. This is the first report of IRES activity in a fish virus and can give us tools to generate antivirals to attack the virus without affecting fish directly.
传染性胰腺坏死病毒（IPNV）是一种鲑鱼病原体，给水产养殖业造成了重大经济损失。IPNV是一种无包膜病毒，包含两个无帽且无多聚腺苷酸化的双链RNA基因组片段，即RNA - A和RNA - B。病毒蛋白Vpg共价连接到两个片段的5'端。关于其病毒周期，尤其是RNA的翻译，人们了解甚少。通过使用单顺反子和双顺反子报告基因的实验，在这项工作中我们表明，RNA - A的120个核苷酸长的5'-UTR包含一个内部核糖体进入位点（IRES），该位点在体外和鲑鱼细胞中均能高效发挥作用。IRES活性强烈依赖于温度。此外，IRES结构局限于5'UTR，不受病毒编码序列的影响。这是鱼类病毒中IRES活性的首次报道，可为我们提供工具来生成抗病毒药物，以攻击病毒而不直接影响鱼类。
