采用响应面法优化茎生莴苣水溶性多糖，并研究其性质和生物活性。
Optimization of water-soluble polysaccharides from stem lettuce by response surface methodology and study on its characterization and bioactivities.
机构信息
Anhui Province Agricultural Products Processing Engineering Laboratory, Anhui Agricultural University, Hefei 230036, China; Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China.
Anhui Province Agricultural Products Processing Engineering Laboratory, Anhui Agricultural University, Hefei 230036, China; Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China; Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
出版信息
Int J Biol Macromol. 2017 Dec;105(Pt 1):912-923. doi: 10.1016/j.ijbiomac.2017.07.125. Epub 2017 Jul 22.
Stem lettuce is widely consumed as a vegetable in China. It is also used as a traditional Chinese medicine for a long time. Up to now, no information is available for stem lettuce polysaccharide (SLP). In this study, extraction optimization, characterization, and antioxidant activity of SLP was investigated.The maximum SLP yield of 20.89% was obtained under the optimal extraction conditions as follows: extraction temperature 95°C, extraction time 3.3h and ratio of water to material 25mL/g. The sulfate content of SLP was 5.82% and the main monosaccharides were GalA, Gal, and Ara with a mole ratio of 49.2: 23.3: 22.9. FT-IR spectrum and HPGPC result further indicated that SLP is a sulfated polydisperse heterpolysaccharide. Congo-red test and AFM scan indicated that SLP might be branching and intertwining structure with triple helix conformation. Moreover, SLP exhibited potent antioxidant activity and α-amylase inhibitory activity, hence it could be used as a potential natural antioxidant and hypoglycemic agent in medicine or functional food fields.
茎用莴苣在中国被广泛作为蔬菜食用。它也被长期用作传统中药。到目前为止，还没有关于茎用莴苣多糖（SLP）的信息。本研究对 SLP 的提取优化、特性和抗氧化活性进行了研究。在最佳提取条件下，获得了 20.89%的最大 SLP 产量，最佳提取条件为：提取温度 95°C，提取时间 3.3h，料液比为 25mL/g。SLP 的硫酸根含量为 5.82%，主要单糖为 GalA、Gal 和 Ara，摩尔比为 49.2:23.3:22.9。傅里叶变换红外光谱（FT-IR）和高效凝胶渗透色谱（HPGPC）结果进一步表明，SLP 是一种硫酸化的多分散杂多糖。刚果红试验和原子力显微镜（AFM）扫描表明，SLP 可能具有分支和交织的结构，具有三螺旋构象。此外，SLP 表现出很强的抗氧化活性和α-淀粉酶抑制活性，因此它可以作为一种有潜力的天然抗氧化剂和降血糖药物，应用于医学或功能性食品领域。
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