Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Namgu, Busan 48513, Republic of Korea; School of Chemical and Bioengineering, Addis Ababa Institute of Technology, Addis Ababa University, P.O. Box 385, Addis Ababa, Ethiopia.
Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Namgu, Busan 48513, Republic of Korea; Department of Life Science, Silla University, 700 Baegyang-daero, Sasang-gu, Busan 46958, Republic of Korea.
Int J Biol Macromol. 2019 Jan;121:852-861. doi: 10.1016/j.ijbiomac.2018.10.091. Epub 2018 Oct 17.
Bioactive polysaccharide was extracted from Pacific oyster Crassostrea gigas using subcritical water (SW) and the extraction process was optimize by response surface methodology (RSM). The optimum condition was found to be temperature 125.01 °C, extraction time 14.93 min, and liquid to solid ratio of 44.69:1 (ml:g). At this condition, the yield of the C. gigas polysaccharides (CGPs) was found to be 18.66%. The polysaccharide was characterized for its chemical, physical, thermal, and structural properties using HPLC, GPC, XRD, FTIR, TGA, UV-Vis spectroscopy, and NMR and the results of this characterization showed a characteristic feature of a typical polysaccharide. The CGPs was found to be a d-glucan with α-(1 → 4) configuration. The CGPs was also evaluated for its antioxidant, antihypertensive, and hypoglycemic activity and the IC (mg/ml) values were found to be 2.06 ± 0.33, 1.58 ± 0.03, and 2.77 ± 0.01 respectively. The current study demonstrated that SWE could be used as an effective process to extract bioactive polysaccharides from C. gigas.
采用亚临界水（SW）从太平洋牡蛎（Crassostrea gigas）中提取生物活性多糖，并通过响应面法（RSM）对提取工艺进行优化。结果发现，最佳条件为温度 125.01°C，提取时间 14.93min，液固比为 44.69:1（ml：g）。在此条件下，C. gigas 多糖（CGPs）的得率为 18.66%。采用 HPLC、GPC、XRD、FTIR、TGA、UV-Vis 光谱和 NMR 对多糖进行了化学、物理、热和结构特性表征，结果表明其具有典型多糖的特征。CGPs 被鉴定为具有α-（1 → 4）构型的 d-葡聚糖。还对 CGPs 的抗氧化、降血压和降血糖活性进行了评价，IC（mg/ml）值分别为 2.06±0.33、1.58±0.03 和 2.77±0.01。本研究表明，SWE 可用于从 C. gigas 中提取生物活性多糖的有效方法。
