Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
Int J Biol Macromol. 2017 Dec;105(Pt 1):947-955. doi: 10.1016/j.ijbiomac.2017.07.121. Epub 2017 Jul 22.
The present study deals with efficacy evaluation of two cationised polysaccharides - Pullulan PEI and Dextran PEI as gene delivery vector after grafting with vinyl imidazole. Chemical modifications of these polysaccharides were confirmed by FTIR and NMR. Nanoplex formed from dextran PEI imidazole (DPI) were of smaller size and possess greater positive charge as compared to that of pullulan PEI imidazole (PPI). MTT assay carried out in C6 and HeLa cell lines revealed that both PPI and DPI are cytocompatible and the percentage cell viability is higher for former. Though DPI and PPI derivatives showed similar cellular uptake, the cell morphology was maintained with later and this may due to the high molecular flexibility of pullulan that masks the cytotoxicity of PEI. Similarly transfection studies carried out with p53 plasmid also revealed that PPI derivatives promote more apoptosis in both C6 and HeLa cell lines as compared to that of DPI derivatives. Based on cytocompatibility and transfection efficiency, PPI was found to be a better gene delivery vector that can be further explored for in vivo applications.
本研究探讨了两种阳离子化多糖 - 支链淀粉 PEI 和葡聚糖 PEI 在接枝乙烯基咪唑后的作为基因传递载体的功效评价。通过傅里叶变换红外光谱（FTIR）和核磁共振（NMR）确认了这些多糖的化学修饰。与支链淀粉 PEI 咪唑（PPI）相比，葡聚糖 PEI 咪唑（DPI）形成的纳米复合物具有更小的粒径和更大的正电荷。在 C6 和 HeLa 细胞系中进行的 MTT 测定表明，PPI 和 DPI 均具有细胞相容性，前者的细胞存活率更高。尽管 DPI 和 PPI 衍生物表现出相似的细胞摄取，但后者保持了细胞形态，这可能是由于支链淀粉的高分子柔性掩盖了 PEI 的细胞毒性。同样，用 p53 质粒进行的转染研究也表明，与 DPI 衍生物相比，PPI 衍生物在 C6 和 HeLa 细胞系中促进更多的细胞凋亡。基于细胞相容性和转染效率，发现 PPI 是一种更好的基因传递载体，可以进一步探索其在体内应用。
