Evivie Smith E, Li Bailiang, Ding Xiuyun, Meng Yueyue, Yu Shangfu, Du Jincheng, Xu Min, Li Wan, Jin Da, Huo Guicheng, Liu Fei
Key Laboratory of Dairy Science, Ministry of Education, College of Food Sciences, Northeast Agricultural UniversityHarbin, China.
Food Science and Nutrition Unit, Department of Animal Science, Faculty of Agriculture, University of BeninBenin City, Nigeria.
Front Microbiol. 2017 Jul 11;8:1238. doi: 10.3389/fmicb.2017.01238. eCollection 2017.
Lactic acid bacteria play increasingly important roles in the food industry. KLDS 3.1003 strain was isolated from traditional yogurt in Inner Mongolia, China. It has shown high antimicrobial activity against selected foodborne and vaginal pathogens. In this study, we investigated and analyzed its complete genome sequence. The KLDS 3.1003 genome comprise of a 1,899,956 bp chromosome with a G+C content of 38.92%, 1,995 genes, and 6 rRNAs. With the exception of M17TZA496, KLDS 3.1003 has more tRNAs (amino acid coding genes) compared to some strains available on the National Centre for Biotechnology Information database. MG-RAST annotation showed that this strain has 317 subsystems with most genes associated with amino acid and carbohydrate metabolism. This strain also has a unique EPS gene cluster containing 23 genes, and may be a mixed dairy starter culture. This information provides more insight into the molecular basis of its potentials for further applications in the dairy and allied industries.
乳酸菌在食品工业中发挥着越来越重要的作用。KLDS 3.1003菌株是从中国内蒙古的传统酸奶中分离出来的。它对某些食源性病原体和阴道病原体表现出高抗菌活性。在本研究中，我们对其全基因组序列进行了调查和分析。KLDS 3.1003基因组由一条1,899,956 bp的染色体组成，G+C含量为38.92%，有1,995个基因和6个rRNA。与美国国立生物技术信息中心数据库中的一些菌株相比，除了M17TZA496外，KLDS 3.1003有更多的tRNA（氨基酸编码基因）。MG-RAST注释显示，该菌株有317个亚系统，大多数基因与氨基酸和碳水化合物代谢相关。该菌株还具有一个独特的包含23个基因的EPS基因簇，可能是一种混合乳制品发酵剂培养物。这些信息为其在乳制品及相关行业进一步应用潜力的分子基础提供了更多见解。
