Li Siqian, Shah Nagendra P
Food and Nutritional Sciences, School of Biological Sciences, The Univ. of Hong Kong, Pokfulam Road, Hong Kong.
J Food Sci. 2016 May;81(5):M1167-76. doi: 10.1111/1750-3841.13276. Epub 2016 Mar 24.
Exo-polysaccharides (EPS) isolated from Streptococcus thermophilus ASCC 1275 were sulfated (31%). High-performance liquid chromatography identified that EPS was composed of mannose (30.19%), galactose (20.10%), glucose (18.05%), glucosamine (16.04%), galactosamine (9.06%), glucuronic acid (3.55%), and ribose (3.01%). Pro-/anti-inflammatory cytokine secretion ratios (IL-1β/IL-10, IL-6/IL-10, and TNF-α/IL-10) of lipopolysaccharide stimulated RAW 264.7 macrophages were significantly decreased by EPS and S.EPS treatments in a dose dependent manner. Furthermore, anti-inflammatory activities of S.EPS improved 49.3% and 24.0% than those of EPS before or after LPS treatment. The reactive oxygen species were inhibited by EPS and S.EPS by 49.6% and 55.1% at 50 μg/mL, respectively. Inhibition activities of S.EPS on nitric oxide production were 12.9% and 55.4% higher than those of EPS at 10 and 50 μg/mL. Additionally, S.EPS exhibited stronger antiproliferative activity on Caco-2 and HepG2 cells. Our results indicated that anti-inflammatory and antiproliferative activities of EPS were significantly (P < 0.01) improved by sulfonation.
从嗜热链球菌ASCC 1275中分离出的胞外多糖（EPS）被硫酸化（硫酸化率为31%）。高效液相色谱法鉴定出EPS由甘露糖（30.19%）、半乳糖（20.10%）、葡萄糖（18.05%）、葡萄糖胺（16.04%）、半乳糖胺（9.06%）、葡萄糖醛酸（3.55%）和核糖（3.01%）组成。经脂多糖刺激的RAW 264.7巨噬细胞的促炎/抗炎细胞因子分泌比率（IL-1β/IL-10、IL-6/IL-10和TNF-α/IL-10）经EPS和硫酸化胞外多糖（S.EPS）处理后呈剂量依赖性显著降低。此外，在脂多糖处理前后，S.EPS的抗炎活性比EPS分别提高了49.3%和24.0%。在50 μg/mL浓度下，EPS和S.EPS对活性氧的抑制率分别为49.6%和55.1%。在10和50 μg/mL浓度下，S.EPS对一氧化氮产生的抑制活性比EPS分别高12.9%和55.4%。此外，S.EPS对Caco-2和HepG2细胞表现出更强的抗增殖活性。我们的结果表明，硫酸化显著（P < 0.01）提高了EPS的抗炎和抗增殖活性。
