壳聚糖衍生物N-[(2-羟基-3-三甲基铵)丙基]氯化物对豚鼠血浆抗凝活性的影响

Effects of Chitosan Derivative N-[(2-Hydroxy-3-Trimethylammonium)Propyl]Chloride on Anticoagulant Activity of Guinea Pig Plasma.

作者信息

Drozd N N, Shagdarova B Ts, Il'ina A V, Varlamov V P

机构信息

National Research Center for Hematology, Ministry of Health of the Russian Federation, Moscow, Russia.

Institute of Bioengineering, Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2017 Jul;163(3):340-343. doi: 10.1007/s10517-017-3799-6. Epub 2017 Jul 26.

DOI:10.1007/s10517-017-3799-6

PMID:28744652

Abstract

Intravenous injection of protamine sulfate or quarternized chitosan derivative to guinea pigs after injection of 70 aIIa U/kg non-fractionated heparin shortened plasma clotting time (shown by partial activated thromboplastin time, thrombin time, and prothrombin time). Intravenous injection of protamine sulfate or quarternized chitosan derivative to guinea pigs after injection of 1 mg/kg (100 aXa U/kg) low-molecular-weight heparin (clexane) led to shortening of plasma clotting time in the ReaClot Heparin test and to prolongation of plasma amidolytic activity in the factor Xa chromogenic substrate test.

摘要

给豚鼠注射70抗Xa国际单位/千克的未分级肝素后，静脉注射硫酸鱼精蛋白或季铵化壳聚糖衍生物可缩短血浆凝血时间（通过部分活化凝血活酶时间、凝血酶时间和凝血酶原时间显示）。给豚鼠注射1毫克/千克（100抗Xa国际单位/千克）的低分子量肝素（克赛）后，静脉注射硫酸鱼精蛋白或季铵化壳聚糖衍生物，在ReaClot肝素试验中会导致血浆凝血时间缩短，在Xa因子显色底物试验中会导致血浆酰胺水解活性延长。