Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.
J Pharm Pharmacol. 2017 Nov;69(11):1615-1624. doi: 10.1111/jphp.12785. Epub 2017 Jul 26.
Some species of the genus Mimosa showed promising results in previous investigations, which include diuretic effect; however, no chemical analyses or animal model has been conducted so far to evaluate the biological properties of M. bimucronata.
Male Wistar rats received the oral treatment with vehicle; hydrochlorothiazide; methanolic extract from M. bimucronata (MEMB), dichloromethane (DCM) and ethyl acetate (EA) fractions or methyl gallate (MG). The cumulative urine volume, electrolytes excretion, pH and osmolality were determined at the end of the experiment.
The chemical studies demonstrated that the phenolic compounds are the majorities in the plant, with the MG being the main substance identified. We showed that MEMB and EA fraction, but not DCM, exhibited diuretic and saluretic effects. Similarly, the MG also revealed diuretic, natriuretic and kaliuretic properties to both normotensive and spontaneously hypertensive rats. Atropine, a muscarinic receptor antagonist, fully prevented MG-induced diuresis and saluresis. In addition, MG did not alter the viability of A7r5 and L929 cell lines and neither stimulated nitric oxide generation.
These findings suggest that M. bimucronata extracts and its majority compound MG present diuretic, natriuretic and kaliuretic properties, which was dependent on the activation of muscarinic acetylcholine receptor.
先前的研究表明，含羞草属的某些物种具有显著的效果，包括利尿作用；然而，迄今为止，尚未进行化学分析或动物模型来评估双齿含羞草的生物特性。
雄性 Wistar 大鼠接受了 vehicle；氢氯噻嗪；双齿含羞草（MEMB）、二氯甲烷（DCM）和乙酸乙酯（EA）馏分或没食子酸甲酯（MG）的口服处理。在实验结束时测定累积尿量、电解质排泄、pH 值和渗透压。
化学研究表明，该植物中的酚类化合物占大多数，其中 MG 是鉴定出的主要物质。我们表明 MEMB 和 EA 馏分，但不是 DCM，具有利尿和利盐作用。同样，MG 对正常血压和自发性高血压大鼠也具有利尿、排钠和排钾作用。毒蕈碱受体拮抗剂阿托品完全阻止了 MG 诱导的利尿和利盐作用。此外，MG 不改变 A7r5 和 L929 细胞系的活力，也不刺激一氧化氮的产生。
这些发现表明，双齿含羞草提取物及其主要化合物 MG 具有利尿、排钠和排钾作用，这依赖于毒蕈碱乙酰胆碱受体的激活。
